Mechanical properties of bacterial cellulose and interactions with smooth muscle cells
Journal article, 2006
Tissue engineered blood vessels (TEBV) represent an attractive approach for overcoming reconstructive problems associated with vascular diseases by providing small calibre vascular grafts. The aim of this study has been to evaluate a novel biomaterial, bacterial cellulose (BC), as a potential scaffold for TEBV. The morphology of the BC pellicle grown in static culture was investigated with SEM. Mechanical properties of BC were measured in Krebs solution and compared with the properties of porcine carotid arteries and ePTFE grafts. Attachment, proliferation and ingrowth of human smooth muscle cells (SMC) on the BC were analysed in vitro. The BC pellicle had an asymmetric structure composed of a fine network of nanofibrils similar to a collagen network. The shape of the stress-strain response of BC is reminiscent of the stress-strain response of the carotid artery, most probably due to the similarity in architecture of the nanofibrill networks. SMC adhered to and proliferated on the BC pellicle; an ingrowth of up to 40 microm was seen after 2 weeks of culture. BC exhibit attractive properties for use in future TEBV.
Animals
Swine
Gluconacetobacter xylinus/metabolism
Cellulose/*pharmacology/*ultrastructure
Cells
Collagen/pharmacology/ultrastructure
Smooth Muscle/cytology/*drug effects
Cell Adhesion
Adhesins
Tissue Engineering
Tensile Strength
Cultured
Cell Proliferation
*Blood Vessel Prosthesis
Bacterial/*pharmacology/*ultrastructure
Carotid Arteries/cytology
Myocytes
Author
Henrik Bäckdahl
Chalmers, Chemical and Biological Engineering, Polymer Technology
Gisela Helenius
University of Gothenburg
Aase Katarina Bodin
Chalmers, Chemical and Biological Engineering, Polymer Technology
Ulf Nannmark
University of Gothenburg
Bengt R Johansson
University of Gothenburg
Bo Risberg
University of Gothenburg
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
Biomaterials
0142-9612 (ISSN) 18785905 (eISSN)
Vol. 27 9 2141-2149Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1016/j.biomaterials.2005.10.026
PubMed
16310848