Identification and DNA annotation of a plasmid isolated from Chromobacterium violaceum
Journal article, 2018

Chromobacterium violaceum is a ß-proteobacterium found widely worldwide with important biotechnological properties and is associated to lethal sepsis in immune-depressed individuals. In this work, we report the discover, complete sequence and annotation of a plasmid detected in C. violaceum that has been unnoticed until now. We used DNA single-molecule analysis to confirm that the episome found was a circular molecule and then proceeded with NGS sequencing. After DNA annotation, we found that this extra-chromosomal DNA is probably a defective bacteriophage of approximately 44 kilobases, with 39 ORFs comprising, mostly hypothetical proteins. We also found DNA sequences that ensure proper plasmid replication and partitioning as well as a toxin addiction system. This report sheds light on the biology of this important species, helping us to understand the mechanisms by which C. violaceum endures to several harsh conditions. This discovery could also be a first step in the development of a DNA manipulation tool in this bacterium.

Author

Daniel C. Lima

Instituto Federal de Educação, Ciência e Tecnologia

Federal University of Rio Grande do Norte

Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Silvia R.Batistuzzo De Medeiros

Federal University of Rio Grande do Norte

Scientific Reports

2045-2322 (ISSN)

Vol. 8 1 5327

Subject Categories

Biophysics

Bioinformatics and Systems Biology

Other Industrial Biotechnology

DOI

10.1038/s41598-018-23708-5

More information

Latest update

4/12/2018