Supercritical fluid extraction of bioactive compounds from berry seeds
Licentiate thesis, 2018
The aim of this study was to investigate the yield and chemical composition of oils obtained by supercritical fluid extraction (SFE) from cloudberry, black currant, and bilberry seeds. The extractions were carried out at different pressures (200–500 bar) and temperatures (40°–80°C). Hexane extraction was performed as a reference.
The berry seed matrices had significant impacts on the extraction efficiency. As black currant seeds have small cells, the milling step resulted in smaller particles with larger surface area, thereby favouring the extraction. The increase in pressure at the same temperature enhanced the supercritical carbon dioxide (SC-CO2) density, resulting in higher extraction yields. Increasing the temperature at 350 bar resulted in the higher levels of vitamin E and carotenoids in the recovered cloudberry and bilberry seed oils. The opposite effect was observed for black currants, due to differences in the seed matrix. The fatty acid composition of black currant seed oil, which includes stearidonic and γ-linolenic acid, suggests that this oil is a good source of polyunsaturated fatty acid. The low ω6/ω3 ratios (1.0–1.3) of cloudberry and black currant seed oils indicate that these oils could be interesting alternatives to fish oil supplements. In addition, the high levels of vitamin E seen in these oils suggest protection against lipid oxidation.
Berry seed oils
Supercritical CO2 extraction
Graziele Gustinelli Carvalho
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Gustinelli, G., Eliasson, L., Svelander, C., Andlid, T., Lundin, L., Ahrné, L., Alminger, M. Supercritical fluid extraction of berry seeds: chemical composition and antioxidant activity
Chemical Process Engineering
Chalmers University of Technology
Utsikten room 7033, Kemihuset floor 7
Opponent: Maud Langton, Swedish University Agricultural science, Uppsala