Metabolic engineering of yeast for fermentative production of flavonoids
Journal article, 2017

Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 +/- 2.66 mg L-1 and quercetin 20.38 +/- 2.57 mg L-1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.

Resokaempferol

Saccharomyces cerevisiae

Fisetin

Kaempferol

Flavonoids

Author

A. Rodriguez

T. Strucko

S. G. Stahlhut

M. Kristensen

D. K. Svenssen

J. Forster

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

I. Borodina

Bioresource Technology

0960-8524 (ISSN)

Vol. 245 1645-1654

Subject Categories

Microbiology

DOI

10.1016/j.biortech.2017.06.043

PubMed

28634125

More information

Latest update

6/8/2018 8