Semi-continuous reverse membrane bioreactor in two-stage anaerobic digestion of citruswaste
Journal article, 2018

The presence of an antimicrobial compound called D-Limonene in citrus waste inhibits methane production from such waste in anaerobic digestion. In this work, a two-stage anaerobic digestion method is developed using reverse membrane bioreactors (rMBRs) containing cells encased in hydrophilic membranes. The purpose of encasement is to retain a high cell concentration inside the bioreactor. The effectiveness of rMBRs in reducing cell washout is evaluated. Three different system configurations, comprising rMBRs, freely suspended cells (FCs), and a combination of both (abbreviated to rMBR-FCs), are incubated at three different organic loading rates (OLRs) each, namely 0.6, 1.2, and 3.6 g COD/(L cycle). Incubation lasts for eight feeding cycles at 55 °C. Methane yield and biogas composition results show that rMBRs perform better than rMBR-FCs and FCs at all three OLRs. Volatile fatty acid profiles and H2production show that the reactors are working properly and no upset occurs. Additionally, a short digestion time of 4 days can be achieved using the rMBR configuration in this study.

Anaerobic digestion

Citrus waste


Membrane bioreactor




Tonny Kurniawan

Gadjah Mada University

Lukitawesa Lukitawesa

University of Borås

Ilma Hanifah

Gadjah Mada University

Rahma wikandari

Gadjah Mada University

Ria Millati

Gadjah Mada University

Mohammad Taherzadeh Esfahani

University of Borås

Claes Niklasson

Chemical Process and Reaction Engineering


1996-1944 (ISSN)

Vol. 11 8 1341

Driving Forces

Sustainable development

Subject Categories

Cell Biology

Renewable Bioenergy Research

Other Industrial Biotechnology

Areas of Advance




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