The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties
Journal article, 2018

Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.

glyceryl ferulate

feruloyl esterase

antioxidants

prenyl caffeate

phylogenetic classification

prenyl ferulate

sugar esters

butyl ferulate

transesterification

l-arabinose ferulate

Author

Gabriella Cerullo

Universita degli Studi di Napoli Federico II

Silvia Hüttner

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Stefan Uthoff

University of Münster

Adiphol Dilokpimol

Utrecht University

Laura Iancu

Dupont Industrial Biosciences

Simona Varriale

Universita degli Studi di Napoli Federico II

Io Antonopoulou

Luleå University of Technology

Miia Mäkelä

University of Helsinki

Vincenza Faraco

Universita degli Studi di Napoli Federico II

Kristiina Hildén

University of Helsinki

Alexander Steinbüchel

King Abdulaziz University

University of Münster

Peter Jütten

Taros Chemicals Gmbh & Co Kg

Alexander Piechot

Taros Chemicals Gmbh & Co Kg

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Ronald P. de Vries

Utrecht University

Ulrika Rova

Luleå University of Technology

Paul Christakopoulos

Luleå University of Technology

Catalysts

2073-4344 (ISSN)

Vol. 8 242

Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)

European Commission (FP7), 2013-12-01 -- 2017-11-30.

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Subject Categories

Bioprocess Technology

Biocatalysis and Enzyme Technology

Organic Chemistry

DOI

10.3390/catal8060242

More information

Latest update

12/19/2018