The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties
Journal article, 2018
Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.
glyceryl ferulate
feruloyl esterase
antioxidants
prenyl caffeate
phylogenetic classification
prenyl ferulate
sugar esters
butyl ferulate
transesterification
l-arabinose ferulate
Author
Gabriella Cerullo
University of Naples Federico II
Silvia Hüttner
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Stefan Uthoff
University of Münster
Adiphol Dilokpimol
Utrecht University
Laura Iancu
Dupont Industrial Biosciences
Simona Varriale
University of Naples Federico II
Io Antonopoulou
Luleå University of Technology
Miia Mäkelä
University of Helsinki
Vincenza Faraco
University of Naples Federico II
Kristiina Hildén
University of Helsinki
Alexander Steinbüchel
King Abdulaziz University
University of Münster
Peter Jütten
Taros Chemicals Gmbh & Co Kg
Alexander Piechot
Taros Chemicals Gmbh & Co Kg
Lisbeth Olsson
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Ronald P. de Vries
Utrecht University
Ulrika Rova
Luleå University of Technology
Paul Christakopoulos
Luleå University of Technology
Catalysts
20734344 (eISSN)
Vol. 8 242 242Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)
European Commission (EC) (EC/FP7/613868), 2013-12-01 -- 2017-11-30.
Areas of Advance
Energy
Life Science Engineering (2010-2018)
Subject Categories
Bioprocess Technology
Biocatalysis and Enzyme Technology
Organic Chemistry
DOI
10.3390/catal8060242