The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties
Artikel i vetenskaplig tidskrift, 2018

Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.

l-arabinose ferulate

sugar esters

transesterification

glyceryl ferulate

phylogenetic classification

butyl ferulate

feruloyl esterase

prenyl caffeate

prenyl ferulate

antioxidants

Författare

Gabriella Cerullo

Universita degli Studi di Napoli Federico II

Silvia Hüttner

Chalmers, Biologi och bioteknik, Industriell bioteknik

Stefan Uthoff

Universität Münster

Adiphol Dilokpimol

Universiteit Utrecht

Laura Iancu

Dupont Industrial Biosciences

Simona Varriale

Universita degli Studi di Napoli Federico II

Io Antonopoulou

Luleå tekniska universitet

Miia Mäkelä

Helsingin Yliopisto

Vincenza Faraco

Universita degli Studi di Napoli Federico II

Kristiina Hildén

Helsingin Yliopisto

Alexander Steinbüchel

King Abdulaziz University

Universität Münster

Peter Jütten

Taros Chemicals Gmbh & Co Kg

Alexander Piechot

Taros Chemicals Gmbh & Co Kg

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Ronald P. de Vries

Universiteit Utrecht

Ulrika Rova

Luleå tekniska universitet

Paul Christakopoulos

Luleå tekniska universitet

Vol. 8 242

Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)

Europeiska kommissionen (FP7), 2013-12-01 -- 2017-11-30.

Styrkeområden

Energi

Livsvetenskaper och teknik

Ämneskategorier

Bioprocessteknik

Biokatalys och enzymteknik

Organisk kemi

DOI

10.3390/catal8060242

Mer information

Senast uppdaterat

2018-08-29