The Synthetic Potential of Fungal Feruloyl Esterases: A Correlation with Current Classification Systems and Predicted Structural Properties
Artikel i vetenskaplig tidskrift, 2018
Twenty-eight fungal feruloyl esterases (FAEs) were evaluated for their synthetic abilities in a ternary system of n-hexane: t-butanol: 100 mM MOPS-NaOH pH 6.0 forming detergentless microemulsions. Five main derivatives were synthesized, namely prenyl ferulate, prenyl caffeate, butyl ferulate, glyceryl ferulate, and l-arabinose ferulate, offering, in general, higher yields when more hydrophilic alcohol substitutions were used. Acetyl xylan esterase-related FAEs belonging to phylogenetic subfamilies (SF) 5 and 6 showed increased synthetic yields among tested enzymes. In particular, it was shown that FAEs belonging to SF6 generally transesterified aliphatic alcohols more efficiently while SF5 members preferred bulkier l-arabinose. Predicted surface properties and structural characteristics were correlated with the synthetic potential of selected tannase-related, acetyl-xylan-related, and lipase-related FAEs (SF1-2, -6, -7 members) based on homology modeling and small molecular docking simulations.
glyceryl ferulate
feruloyl esterase
antioxidants
prenyl caffeate
phylogenetic classification
prenyl ferulate
sugar esters
butyl ferulate
transesterification
l-arabinose ferulate
Författare
Gabriella Cerullo
Universita degli Studi di Napoli Federico II
Silvia Hüttner
Chalmers, Biologi och bioteknik, Industriell bioteknik
Stefan Uthoff
Universität Münster
Adiphol Dilokpimol
Universiteit Utrecht
Laura Iancu
Dupont Industrial Biosciences
Simona Varriale
Universita degli Studi di Napoli Federico II
Io Antonopoulou
Luleå tekniska universitet
Miia Mäkelä
Helsingin Yliopisto
Vincenza Faraco
Universita degli Studi di Napoli Federico II
Kristiina Hildén
Helsingin Yliopisto
Alexander Steinbüchel
King Abdulaziz University
Universität Münster
Peter Jütten
Taros Chemicals Gmbh & Co Kg
Alexander Piechot
Taros Chemicals Gmbh & Co Kg
Lisbeth Olsson
Chalmers, Biologi och bioteknik, Industriell bioteknik
Ronald P. de Vries
Universiteit Utrecht
Ulrika Rova
Luleå tekniska universitet
Paul Christakopoulos
Luleå tekniska universitet
Catalysts
20734344 (eISSN)
Vol. 8 242 242Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)
Europeiska kommissionen (EU) (EC/FP7/613868), 2013-12-01 -- 2017-11-30.
Styrkeområden
Energi
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Bioprocessteknik
Biokatalys och enzymteknik
Organisk kemi
DOI
10.3390/catal8060242