Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)
Forskningsprojekt , 2013 – 2017

OPTIBIOCAT is a 48 months project aimed at developing biocatalysts based on feruloyl esterases (FAEs) and glucuronoyl esterases (GEs) for production of phenolic fatty- and sugar- esters with antioxidant activity for cosmetic industry, expanding the number/type of industrial biotransformations. Selected FAEs and GEs available within the consortium will be improved for their thermo- and solvent- resistance and substrate specificity by site-directed mutagenesis and directed evolution. Novel enzymes will be discovered by mining for new genes from available genomes. An inventory of novel FAEs and GEs will be developed including 50 fungal and 500 bacterial esterases, 25 site-directed and 20 directed evolved mutants. Enzymatic performances will be optimized to enhance the yield (up to the theoretical yield of 100%) and productivity (up to 0.5-1 g/l/h) of reactions giving the main targeted antioxidants: butyl ferulate, p-coumarate, caffeate, sinapate and 5-O-(trans-feruloyl)-arabinofuranose (using FAEs), glucuronate and benzyl glucuronate (using GEs). FAEs and GEs will be also tested for production of other compounds with improved biological activity and properties of hydrophilicity/hydrophobicity for cosmetic applications. Cost-effective methods will be developed for production of the new biocatalysts, in the g/L scale, and for their technical application to produce antioxidants for cosmetic industry, up to 20L. Enzyme immobilization will increase their recyclability up to ten cycles. The ability of the developed catalysts to work in conditions miming the industrial ones with reduced use of solvents and lower temperature than the chemical routes will be demonstrated. The techno-economic viability and environmental friendliness will be assessed considering a full industrial scale scenario. OPTIBIOCAT involves a highly skilled and multidisciplinary partnership of 16 partners from 8 EU countries, and it is a strongly industry driven project through the participation of 8 SMEs and 1 large company.

Deltagare

Lisbeth Olsson (kontakt)

Avdelningschef vid Industriell Bioteknik

Sun-Li Chong

vid Chalmers, Biologi och bioteknik, Industriell bioteknik

Silvia Hüttner

Forskare vid Chalmers, Biologi och bioteknik, Industriell bioteknik

Samarbetspartners

Biocom

Berlin, Germany

Clea Technologies Bv

Delft, Netherlands

Dyadic Nederland Bv

Wageningen, Netherlands

Helsingin Yliopisto

Helsingin Yliopisto, Finland

Institut national de la recherche agronomique (INRA)

Paris, France

Koninklijke Nederlandse Akademie Van Wetenschappen - Knaw

Amsterdam, Netherlands

Korres S.A. Natural Products (Korres Anonimi Eteria Fysika Proionta)

Inofyta, Greece

Luleå tekniska universitet

Luleå, Sweden

Nzytech Lda

Lisboa, Portugal

Proteonic Bv

Leiden, Netherlands

Servicexs Bv

Leiden, Netherlands

Supren Gmbh

Dortmund, Germany

Taros Chemicals Gmbh & Co Kg

Dortmund, Germany

Universita degli Studi di Napoli Federico II

Napoli, Italy

Universität Münster

Münster, Germany

Finansiering

Europeiska kommissionen (FP7)

Finansierar Chalmers deltagande under 2013–2017

Relaterade styrkeområden och infrastruktur

Hållbar utveckling

Drivkrafter

Produktion

Styrkeområden

Energi

Styrkeområden

Livsvetenskaper och teknik (2010-2018)

Styrkeområden

Publikationer

Mer information

Projektets webbplats

http://www.optibiocat.eu

Senast uppdaterat

2018-02-26