Optimized esterase biocatalysts for cost-effective industrial production (OPTIBIOCAT)
Forskningsprojekt, 2013
– 2017
OPTIBIOCAT is a 48 months project aimed at developing biocatalysts based on feruloyl esterases (FAEs) and glucuronoyl esterases (GEs) for production of phenolic fatty- and sugar- esters with antioxidant activity for cosmetic industry, expanding the number/type of industrial biotransformations. Selected FAEs and GEs available within the consortium will be improved for their thermo- and solvent- resistance and substrate specificity by site-directed mutagenesis and directed evolution. Novel enzymes will be discovered by mining for new genes from available genomes. An inventory of novel FAEs and GEs will be developed including 50 fungal and 500 bacterial esterases, 25 site-directed and 20 directed evolved mutants. Enzymatic performances will be optimized to enhance the yield (up to the theoretical yield of 100%) and productivity (up to 0.5-1 g/l/h) of reactions giving the main targeted antioxidants: butyl ferulate, p-coumarate, caffeate, sinapate and 5-O-(trans-feruloyl)-arabinofuranose (using FAEs), glucuronate and benzyl glucuronate (using GEs). FAEs and GEs will be also tested for production of other compounds with improved biological activity and properties of hydrophilicity/hydrophobicity for cosmetic applications. Cost-effective methods will be developed for production of the new biocatalysts, in the g/L scale, and for their technical application to produce antioxidants for cosmetic industry, up to 20L. Enzyme immobilization will increase their recyclability up to ten cycles. The ability of the developed catalysts to work in conditions miming the industrial ones with reduced use of solvents and lower temperature than the chemical routes will be demonstrated. The techno-economic viability and environmental friendliness will be assessed considering a full industrial scale scenario. OPTIBIOCAT involves a highly skilled and multidisciplinary partnership of 16 partners from 8 EU countries, and it is a strongly industry driven project through the participation of 8 SMEs and 1 large company.
Deltagare
Lisbeth Olsson (kontakt)
Industriell Bioteknik
Sun-Li Chong
Chalmers, Life sciences, Industriell bioteknik
Silvia Hüttner
Chalmers, Life sciences, Industriell bioteknik
Samarbetspartners
Biocom
Berlin, Germany
Clea Technologies Bv
Delft, Netherlands
Dyadic Nederland Bv
Wageningen, Netherlands
Helsingin Yliopisto
Helsinki, Finland
Institut national de la recherche agronomique (INRA)
Paris, France
Koninklijke Nederlandse Akademie Van Wetenschappen - Knaw
Amsterdam, Netherlands
Korres S.A. Natural Products (Korres Anonimi Eteria Fysika Proionta)
Inofyta, Greece
Luleå tekniska universitet
Luleå, Sweden
Nzytech Lda
Lisboa, Portugal
Proteonic Bv
Leiden, Netherlands
Servicexs Bv
Leiden, Netherlands
Supren Gmbh
Dortmund, Germany
Taros Chemicals Gmbh & Co Kg
Dortmund, Germany
Universita degli Studi di Napoli Federico II
Napoli, Italy
Universität Münster
Münster, Germany
Finansiering
Europeiska kommissionen (EU)
Projekt-id: EC/FP7/613868
Finansierar Chalmers deltagande under 2013–2017
Relaterade styrkeområden och infrastruktur
Hållbar utveckling
Drivkrafter
Produktion
Styrkeområden
Energi
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
Styrkeområden