Biomimetic graphene for enhanced interaction with the external membrane of astrocytes
Journal article, 2018

Graphene and graphene substrates display huge potential as material interfaces for devices and biomedical tools targeting the modulation or recovery of brain functionality. However, to be considered reliable neural interfaces, graphene-derived substrates should properly interact with astrocytes, favoring their growth and avoiding adverse gliotic reactions. Indeed, astrocytes are the most abundant cells in the human brain and they have a crucial physiological role to maintain its homeostasis and modulate synaptic transmission. In this work, we describe a new strategy based on the chemical modification of graphene oxide (GO) with a synthetic phospholipid (PL) to improve interaction of GO with brain astroglial cells. The PL moieties were grafted on GO sheets through polymeric brushes obtained by atom-transfer radical-polymerization (ATRP) between acryloyl-modified PL and GO nanosheets modified with a bromide initiator. The adhesion of primary rat cortical astrocytes on GO-PL substrates increased by about three times with respect to that on glass substrates coated with standard adhesion agents (i.e. poly-d-lysine, PDL) as well as with respect to that on non-functionalized GO. Moreover, we show that astrocytes seeded on GO-PL did not display significant gliotic reactivity, indicating that the material interface did not cause a detrimental inflammatory reaction when interacting with astroglial cells. Our results indicate that the reported biomimetic approach could be applied to neural prosthesis to improve cell colonization and avoid glial scar formation in brain implants. Additionally, improved adhesion could be extremely relevant in devices targeting neural cell sensing/modulation of physiological activity.

Author

M. Durso

Institute for organic syntheses and photoreactivity (ISOF-CNR)

A. I. Borrachero-Conejo

Istituto Per Lo Studio Dei Materiali Nanostrutturati, Rome

C. Bettini

Institute for organic syntheses and photoreactivity (ISOF-CNR)

E. Treossi

Institute for organic syntheses and photoreactivity (ISOF-CNR)

A. Scidà

Institute for organic syntheses and photoreactivity (ISOF-CNR)

E. Saracino

Institute for organic syntheses and photoreactivity (ISOF-CNR)

M. Gazzano

Institute for organic syntheses and photoreactivity (ISOF-CNR)

Meganne Christian

Consiglo Nazionale Delle Richerche

V. Morandi

Consiglo Nazionale Delle Richerche

G. Tuci

Consiglo Nazionale Delle Richerche

G. Giambastiani

Consiglo Nazionale Delle Richerche

Kazan Federal University

L. Ottaviano

CNR - SuPerconducting and other INnovative materials and devices institute, L`Aquila

University of L'Aquila

F. Perrozzi

CNR - SuPerconducting and other INnovative materials and devices institute, L`Aquila

V. Benfenati

Institute for organic syntheses and photoreactivity (ISOF-CNR)

M. Melucci

Institute for organic syntheses and photoreactivity (ISOF-CNR)

Vincenzo Palermo

Institute for organic syntheses and photoreactivity (ISOF-CNR)

Chalmers, Industrial and Materials Science, Materials and manufacture

Journal of Materials Chemistry B

20507518 (ISSN) 2050750X (eISSN)

Vol. 6 33 5335-5342

Subject Categories

Materials Engineering

Neurosciences

Cell and Molecular Biology

Biomaterials Science

DOI

10.1039/c8tb01410h

More information

Latest update

9/18/2018