Biomimetic graphene for enhanced interaction with the external membrane of astrocytes
Artikel i vetenskaplig tidskrift, 2018
Graphene and graphene substrates display huge potential as material interfaces for devices and biomedical tools targeting the modulation or recovery of brain functionality. However, to be considered reliable neural interfaces, graphene-derived substrates should properly interact with astrocytes, favoring their growth and avoiding adverse gliotic reactions. Indeed, astrocytes are the most abundant cells in the human brain and they have a crucial physiological role to maintain its homeostasis and modulate synaptic transmission. In this work, we describe a new strategy based on the chemical modification of graphene oxide (GO) with a synthetic phospholipid (PL) to improve interaction of GO with brain astroglial cells. The PL moieties were grafted on GO sheets through polymeric brushes obtained by atom-transfer radical-polymerization (ATRP) between acryloyl-modified PL and GO nanosheets modified with a bromide initiator. The adhesion of primary rat cortical astrocytes on GO-PL substrates increased by about three times with respect to that on glass substrates coated with standard adhesion agents (i.e. poly-d-lysine, PDL) as well as with respect to that on non-functionalized GO. Moreover, we show that astrocytes seeded on GO-PL did not display significant gliotic reactivity, indicating that the material interface did not cause a detrimental inflammatory reaction when interacting with astroglial cells. Our results indicate that the reported biomimetic approach could be applied to neural prosthesis to improve cell colonization and avoid glial scar formation in brain implants. Additionally, improved adhesion could be extremely relevant in devices targeting neural cell sensing/modulation of physiological activity.
Författare
M. Durso
Consiglio Nazionale delle Ricerche (CNR)
A. I. Borrachero-Conejo
Istituto per lo Studio dei Materiali Nanostrutturati del C.N.R.
C. Bettini
Consiglio Nazionale delle Ricerche (CNR)
E. Treossi
Consiglio Nazionale delle Ricerche (CNR)
A. Scidà
Consiglio Nazionale delle Ricerche (CNR)
E. Saracino
Consiglio Nazionale delle Ricerche (CNR)
M. Gazzano
Consiglio Nazionale delle Ricerche (CNR)
Meganne Christian
Consiglo Nazionale Delle Richerche
V. Morandi
Consiglo Nazionale Delle Richerche
G. Tuci
Consiglo Nazionale Delle Richerche
G. Giambastiani
Kazan Federal University
Consiglo Nazionale Delle Richerche
L. Ottaviano
Consiglio Nazionale delle Ricerche (CNR)
Universita degli Studi dell'Aquila
F. Perrozzi
Consiglio Nazionale delle Ricerche (CNR)
V. Benfenati
Consiglio Nazionale delle Ricerche (CNR)
M. Melucci
Consiglio Nazionale delle Ricerche (CNR)
Vincenzo Palermo
Consiglio Nazionale delle Ricerche (CNR)
Chalmers, Industri- och materialvetenskap, Material och tillverkning
Journal of Materials Chemistry B
2050750x (ISSN) 20507518 (eISSN)
Vol. 6 33 5335-5342Ämneskategorier (SSIF 2011)
Materialteknik
Neurovetenskaper
Cell- och molekylärbiologi
Biomaterialvetenskap
DOI
10.1039/c8tb01410h