Tailormade Polysaccharides with Defined Branching Patterns: Enzymatic Polymerization of Arabinoxylan Oligosaccharides
Journal article, 2018
The heterogeneous nature of non-cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase-catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X-ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure–property relationship studies that are not accessible by other means.
synthetic methods
enzymes
carbohydrates
glycosynthases
structure elucidation
Author
Deborah Senf
Freie Universität Berlin
Max Planck Society
Colin Ruprecht
Max Planck Society
Saina Kishani
Royal Institute of Technology (KTH)
Aleksandar Matic
University of Potsdam
Max Planck Society
Guillermo Toriz Gonzalez
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology
Paul Gatenholm
Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Paul Gatenholm Group
L. Wagberg
Royal Institute of Technology (KTH)
Fabian Pfrengle
Freie Universität Berlin
Max Planck Society
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 57 37 11987-11992Subject Categories
Textile, Rubber and Polymeric Materials
Other Chemistry Topics
Organic Chemistry
DOI
10.1002/anie.201806871