Tailormade Polysaccharides with Defined Branching Patterns: Enzymatic Polymerization of Arabinoxylan Oligosaccharides
Artikel i vetenskaplig tidskrift, 2018
The heterogeneous nature of non-cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase-catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X-ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure–property relationship studies that are not accessible by other means.
glycosynthases
enzymes
structure elucidation
carbohydrates
synthetic methods
Författare
Deborah Senf
Max-Planck-Gesellschaft
Freie Universität Berlin
Colin Ruprecht
Max-Planck-Gesellschaft
Saina Kishani
Kungliga Tekniska Högskolan (KTH)
Aleksandar Matic
Max-Planck-Gesellschaft
Universität Potsdam
Guillermo Toriz Gonzalez
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Paul Gatenholm
Chalmers, Kemi och kemiteknik, Tillämpad kemi
L. Wagberg
Kungliga Tekniska Högskolan (KTH)
Fabian Pfrengle
Freie Universität Berlin
Max-Planck-Gesellschaft
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 57 37 11987-11992Ämneskategorier (SSIF 2011)
Textil-, gummi- och polymermaterial
Annan kemi
Organisk kemi
DOI
10.1002/anie.201806871