In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink
Journal article, 2019

Bacterial nanocellulose (BNC) is a 3D network of nanofibrils exhibiting excellent biocompatibility. Here, we present the aqueous counter collision (ACC) method of BNC disassembly to create bioink with suitable properties for cartilage-specific 3D-bioprinting. BNC was disentangled by ACC, and fibril characteristics were analyzed. Bioink printing fidelity and shear-thinning properties were evaluated. Cell-laden bioprinted grid constructs (5 × 5 × 1 mm 3 ) containing human nasal chondrocytes (10 M mL -1 ) were implanted in nude mice and explanted after 30 and 60 days. Both ACC and hydrolysis resulted in significantly reduced fiber lengths, with ACC resulting in longer fibrils and fewer negative charges relative to hydrolysis. Moreover, ACC-BNC bioink showed outstanding printability, postprinting mechanical stability, and structural integrity. In vivo, cell-laden structures were rapidly integrated, maintained structural integrity, and showed chondrocyte proliferation, with 32.8 ± 13.8 cells per mm 2 observed after 30 days and 85.6 ± 30.0 cells per mm 2 at day 60 (p = 0.002). Furthermore, a full-thickness skin graft was attached and integrated completely on top of the 3D-bioprinted construct. The novel ACC disentanglement technique makes BNC biomaterial highly suitable for 3D-bioprinting and clinical translation, suggesting cell-laden 3D-bioprinted ACC-BNC as a promising solution for cartilage repair.

neocartilage formation

3D-bioprinting

bacterial nanocellulose

bioinks

aqueous counter collision

Author

Peter Apelgren

Sahlgrenska University Hospital

Erdem Karabulut

BBV at Biotech Center

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Paul Gatenholm Group

Matteo Amoroso

Sahlgrenska University Hospital

Athanasios Mantas

BBV at Biotech Center

Hector Martinez Avila

BBV at Biotech Center

Lars Kölby

Sahlgrenska University Hospital

Tetsuo Kondo

Kyushu University

Guillermo Toriz Gonzalez

University of Guadalajara

Paul Gatenholm

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Paul Gatenholm Group

BBV at Biotech Center

ACS Biomaterial Science and Engineering

2373-9878 (eISSN)

Subject Categories

Bio Materials

Bioengineering Equipment

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1021/acsbiomaterials.9b00157

More information

Latest update

5/13/2019