Electrospray sample injection for single-particle imaging with x-ray lasers
Journal article, 2019

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

Author

Johan Bielecki

European XFEL

Uppsala University

Max F. Hantke

Refeyn Ltd.

University of Oxford

Uppsala University

Benedikt J. Daurer

NUS Centre for Bioimaging Sciences

Uppsala University

Hemanth K.N. Reddy

Uppsala University

Dirk Hasse

Uppsala University

Daniel S.D. Larsson

Uppsala University

Laura H. Gunn

Uppsala University

Martin Svenda

Uppsala University

AlbaNova University Center

Anna Munke

Uppsala University

Jonas A. Sellberg

AlbaNova University Center

Uppsala University

Leonie Flueckiger

La Trobe University

Alberto Pietrini

Uppsala University

Carl Nettelblad

Uppsala University

Ida Lundholm

Uppsala University

Gunilla Carlsson

Uppsala University

Kenta Okamoto

Uppsala University

N. Timneanu

Uppsala University

Daniel Westphal

Uppsala University

Olena Kulyk

Czech Academy of Sciences

Akifumi Higashiura

Osaka University

Hiroshima University

Gijs Van Der Schot

Bijvoet Center for Biomolecular Research

Uppsala University

Ne Te Duane Loh

National University of Singapore (NUS)

NUS Centre for Bioimaging Sciences

Taylor E. Wysong

Stanford University

Christoph Bostedt

Argonne National Laboratory

Northwestern University

Tais Gorkhover

Stanford University

Bianca Iwan

University of Hanover

Stanford University

M. Marvin Seibert

Uppsala University

Timur Osipov

Stanford University

Peter Walter

Stanford University

Philip Hart

Stanford University

Max Bucher

Argonne National Laboratory

Anatoli Ulmer

Technische Universität Berlin

Dipanwita Ray

Stanford University

Gabriella Carini

Stanford University

Brookhaven National Laboratory

Ken Ferguson

Stanford University

Inger Andersson

Uppsala University

Jakob Andreasson

Czech Academy of Sciences

Chalmers, Physics, Materials Physics

Janos Hajdu

Czech Academy of Sciences

Uppsala University

Filipe R.N.C. Maia

Lawrence Berkeley National Laboratory

Uppsala University

Science advances

2375-2548 (eISSN)

Vol. 5 5 eaav8801

Subject Categories

Analytical Chemistry

Atom and Molecular Physics and Optics

Other Physics Topics

DOI

10.1126/sciadv.aav8801

More information

Latest update

8/28/2020