Electrospray sample injection for single-particle imaging with x-ray lasers
Artikel i vetenskaplig tidskrift, 2019

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

Författare

Johan Bielecki

European XFEL

Uppsala universitet

Max F. Hantke

University of Oxford

Refeyn Ltd.

Uppsala universitet

Benedikt J. Daurer

NUS Centre for Bioimaging Sciences

Uppsala universitet

Hemanth K.N. Reddy

Uppsala universitet

Dirk Hasse

Uppsala universitet

Daniel S.D. Larsson

Uppsala universitet

Laura H. Gunn

Uppsala universitet

Martin Svenda

AlbaNova University Center

Uppsala universitet

Anna Munke

Uppsala universitet

Jonas A. Sellberg

AlbaNova University Center

Uppsala universitet

Leonie Flueckiger

La Trobe University

Alberto Pietrini

Uppsala universitet

Carl Nettelblad

Uppsala universitet

Ida Lundholm

Uppsala universitet

Gunilla Carlsson

Uppsala universitet

Kenta Okamoto

Uppsala universitet

N. Timneanu

Uppsala universitet

Daniel Westphal

Uppsala universitet

Olena Kulyk

Czech Academy of Sciences

Akifumi Higashiura

Osaka University

Hiroshima University

Gijs Van Der Schot

Bijvoet Center for Biomolecular Research

Uppsala universitet

Ne Te Duane Loh

Universiti Kebangsaan Singapura (NUS)

NUS Centre for Bioimaging Sciences

Taylor E. Wysong

Stanford University

Christoph Bostedt

Argonne National Laboratory

Northwestern University

Tais Gorkhover

Stanford University

Bianca Iwan

Stanford University

Leibniz Universität Hannover

M. Marvin Seibert

Uppsala universitet

Timur Osipov

Stanford University

Peter Walter

Stanford University

Philip Hart

Stanford University

Max Bucher

Argonne National Laboratory

Anatoli Ulmer

Technische Universität Berlin

Dipanwita Ray

Stanford University

Gabriella Carini

Stanford University

Brookhaven National Laboratory

Ken Ferguson

Stanford University

Inger Andersson

Uppsala universitet

Jakob Andreasson

Czech Academy of Sciences

Chalmers, Fysik, Kondenserade materiens fysik

Janos Hajdu

Uppsala universitet

Czech Academy of Sciences

Filipe R.N.C. Maia

Lawrence Berkeley National Laboratory

Uppsala universitet

Science advances

2375-2548 (eISSN)

Vol. 5 5 eaav8801

Ämneskategorier

Analytisk kemi

Atom- och molekylfysik och optik

Annan fysik

DOI

10.1126/sciadv.aav8801

Mer information

Senast uppdaterat

2019-07-01