Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles
Journal article, 2019

Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high-yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria-based proteoliposomes are an attractive eco-friendly alternative that can outperform currently used liposomes.



click chemistry

bacterial production



Jorge Royes

Paris Diderot University

Ecole Normale Superieure (ENS)

Oana Ilioaia

Paris Diderot University

Quentin Lubart

Chalmers, Physics

Federica Angius

Institute for Water and Wetland Research

Paris Diderot University

Galina V. Dubacheva

Laboratoire Photophysique et Photochimie Supramoléculaires et Macromoléculaires

Marta Bally

Chalmers, Physics, Biological Physics

Bruno Miroux

Paris Diderot University

Christophe Tribet

Ecole Normale Superieure (ENS)

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 58 22 7395-7399

Subject Categories

Other Chemistry Topics

Biocatalysis and Enzyme Technology

Organic Chemistry



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