Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles
Artikel i vetenskaplig tidskrift, 2019

Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high-yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria-based proteoliposomes are an attractive eco-friendly alternative that can outperform currently used liposomes.

encapsulation

proteoliposome

nanovesicle

bacterial production

click chemistry

Författare

Jorge Royes

Université Paris Diderot

Ecole Normale Superieure (ENS)

Oana Ilioaia

Université Paris Diderot

Quentin Lubart

Federica Angius

Université Paris Diderot

Institute for Water and Wetland Research

Galina V. Dubacheva

Laboratoire Photophysique et Photochimie Supramoléculaires et Macromoléculaires

Marta Bally

Chalmers, Fysik, Biologisk fysik

Bruno Miroux

Université Paris Diderot

Christophe Tribet

Ecole Normale Superieure (ENS)

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. 58 22 7395-7399

Ämneskategorier

Annan kemi

Biokatalys och enzymteknik

Organisk kemi

DOI

10.1002/anie.201902929

Mer information

Senast uppdaterat

2019-07-26