Hydrophobic catalysis and a potential biological role of DNA unstacking induced by environment effects
Journal article, 2019

Hydrophobic base stacking is a major contributor to DNA double-helix stability. We report the discovery of specific unstacking effects in certain semihydrophobic environments. Water-miscible ethylene glycol ethers are found to modify structure, dynamics, and reactivity of DNA by mechanisms possibly related to a biologically relevant hydrophobic catalysis. Spectroscopic data and optical tweezers experiments show that base-stacking energies are reduced while base-pair hydrogen bonds are strengthened. We propose that a modulated chemical potential of water can promote “longitudinal breathing” and the formation of unstacked holes while base unpairing is suppressed. Flow linear dichroism in 20% diglyme indicates a 20 to 30% decrease in persistence length of DNA, supported by an increased flexibility in single-molecule nanochannel experiments in poly(ethylene glycol). A limited (3 to 6%) hyperchromicity but unaffected circular dichroism is consistent with transient unstacking events while maintaining an overall average B-DNA conformation. Further information about unstacking dynamics is obtained from the binding kinetics of large thread-intercalating ruthenium complexes, indicating that the hydrophobic effect provides a 10 to 100 times increased DNA unstacking frequency and an “open hole” population on the order of 10−2 compared to 10−4 in normal aqueous solution. Spontaneous DNA strand exchange catalyzed by poly(ethylene glycol) makes us propose that hydrophobic residues in the L2 loop of recombination enzymes RecA and Rad51 may assist gene recombination via modulation of water activity near the DNA helix by hydrophobic interactions, in the manner described here. We speculate that such hydrophobic interactions may have catalytic roles also in other biological contexts, such as in polymerases.

DNA polymerase

Hydrophobic catalysis

DNA

Threading intercalation

RecA

Author

Bobo Feng

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Robert P. Sosa

University of California

Anna Mårtensson

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Kai Jiang

Chalmers, Biology and Biological Engineering, Food and Nutrition Science

Alex Tong

University of California

Kevin D. Dorfman

University of Minnesota

Masayuki Takahashi

Tokyo Institute of Technology

Per Lincoln

Chalmers, Chemistry and Chemical Engineering

C. Bustamante

University of California

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Bengt Nordén

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 116 35 17169-17174

Subject Categories

Physical Chemistry

Biophysics

Structural Biology

DOI

10.1073/pnas.1909122116

PubMed

31413203

More information

Latest update

5/14/2020