Microfluidic technology for investigation of protein function in single adherent cells
Book chapter, 2019

Instrumental techniques and associated methods for single cell analysis, designed to investigate and measure a broad range of cellular parameters in search of unique features, address key limitations of conventional cell-based assays with their ensemble average response. While many different single cell techniques exist for suspension cultures, which can process and characterize large numbers of individual cells in rapid succession, the access to surface-immobilized cells in typical 2D and 3D culture environments remains challenging. Open space microfluidics has created new possibilities in this area, allowing for exclusive access to single cells in adherent cultures, even at high confluency. In this chapter, we briefly review new microtechnologies for the investigation of protein function in single adherent cells, and present an overview over related recent applications of the multifunctional pipette (Biopen), a microfluidic multi-solution dispensing system that uses hydrodynamic confinement in open volume environments in order to establish a superfusion zone over selected single cells in adherent cultures.

Open space microfluidics

Single cell enzymology

Multifunctional pipette

Superfusion

Single cell studies

Biopen

Author

Aldo Jesorka

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

I. Poldsalu

University of Oslo

Irep Gözen

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

University of Oslo

Methods in Enzymology


978-012817090-8 (ISBN)

Subject Categories

Cell and Molecular Biology

Other Medical Biotechnology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1016/bs.mie.2019.07.038

More information

Latest update

11/27/2019