Microfluidic technology for investigation of protein function in single adherent cells
Kapitel i bok, 2019

Instrumental techniques and associated methods for single cell analysis, designed to investigate and measure a broad range of cellular parameters in search of unique features, address key limitations of conventional cell-based assays with their ensemble average response. While many different single cell techniques exist for suspension cultures, which can process and characterize large numbers of individual cells in rapid succession, the access to surface-immobilized cells in typical 2D and 3D culture environments remains challenging. Open space microfluidics has created new possibilities in this area, allowing for exclusive access to single cells in adherent cultures, even at high confluency. In this chapter, we briefly review new microtechnologies for the investigation of protein function in single adherent cells, and present an overview over related recent applications of the multifunctional pipette (Biopen), a microfluidic multi-solution dispensing system that uses hydrodynamic confinement in open volume environments in order to establish a superfusion zone over selected single cells in adherent cultures.

Open space microfluidics

Single cell enzymology

Multifunctional pipette

Superfusion

Single cell studies

Biopen

Författare

Aldo Jesorka

Chalmers, Kemi och kemiteknik, Kemi och biokemi

I. Poldsalu

Universitetet i Oslo

Irep Gözen

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Universitetet i Oslo

Methods in Enzymology

Ämneskategorier

Cell- och molekylärbiologi

Annan medicinsk bioteknologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1016/bs.mie.2019.07.038

Mer information

Senast uppdaterat

2019-11-27