Novel AA9 LPMOs from the thermophilic fungus Thielavia terrestris LPH 172
Conference poster, 2019
improved thermostability. One such organism is the fungus Thielavia terrestris with alfalfa straw hydrolysis optima around 40 and 60°C (Berka
et al., 2011). We isolated the ascomycete T. terrestris strain LPH 172 from compost in Vietnam and fully sequenced its genome. We have also
characterized the strain as acidophilic (growing at pH 1) and thermophilic (50°C). High cellulase and xylanase activities at pH 3 and 70°C were also
observed. Full genome sequencing revealed 10 128 genes, of which according to dbCAN2 380 encode for putative CAZymes and of those 18 for AA9 LPMOs. We also obtained transcriptomics data from growth on five different substrates: glucose, cellulose (Avicel), beechwood xylan, corncob xylan and rice straw. From differential and absolute gene expression data analysis, we have selected nine putative AA9 LPMO enzymes that showed upregulation on growth on either Avicel or rice straw compared to growth at glucose. For further characterization of these nine AA9s, they will be cloned and produced in Pichia pastoris and analyzed for processing simple and complex biomasses.
Author
Monika Tõlgo
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Silvia Hüttner
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Johan Larsbrink
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Lisbeth Olsson
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Thuy Nguyen Thanh
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Thanh Nguyen Vu
Subject Categories
Microbiology
Biocatalysis and Enzyme Technology
Other Industrial Biotechnology