Novel AA9 LPMOs from the thermophilic fungus Thielavia terrestris LPH 172
Conference poster, 2019

For making biomass saccharification more efficient, industry is looking
into thermophilic microorganisms for production of enzymes with
improved thermostability. One such organism is the fungus Thielavia
terrestris with alfalfa straw hydrolysis optima around 40 and 60°C (Berka
et al., 2011). We isolated the ascomycete T. terrestris strain LPH 172 from
compost in Vietnam and fully sequenced its genome. We have also
characterized the strain as acidophilic (growing at pH 1) and thermophilic
(50°C). High cellulase and xylanase activities at pH 3 and 70°C were also
observed. Full genome sequencing revealed 10 128 genes,
of which according to dbCAN2 380 encode for putative CAZymes and of
those 18 for AA9 LPMOs. We also obtained transcriptomics data from
growth on five different substrates: glucose, cellulose (Avicel), beechwood
xylan, corncob xylan and rice straw. From differential and absolute gene
expression data analysis, we have selected nine putative AA9 LPMO
enzymes that showed upregulation on growth on either Avicel or rice straw
compared to growth at glucose. For further characterization of these nine
AA9s, they will be cloned and produced in Pichia pastoris and analyzed for
processing simple and complex biomasses.

Author

Monika Tõlgo

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Silvia Hüttner

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Johan Larsbrink

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Thuy Nguyen Thanh

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Thanh Nguyen Vu

Subject Categories

Microbiology

Biocatalysis and Enzyme Technology

Other Industrial Biotechnology

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