Johan Larsbrink

Associate Professor at Industrial Biotechnology

Johan Larsbrink works as Associate Professor at the Division of Industrial Biotechnology, which he joined in 2015. He obtained his PhD at the Royal Institute of Technology (KTH) in 2013 and afterwards worked as a postdoctoral fellow at the Norwegian University of Life Sciences. He is a member of the , a national multi-university center aiming to create novel materials from wood. In 2019, Johan was awarded the Research Supervisor of the Year Award Board of Graduate Students, and in 2021 he was elected as a member of the Young Academy of Sweden. He is one of the two directors of study at the Bioscience graduate school at Chalmers.

His research background and current interests revolve around microbial deconstruction of complex biomass, in particular the carbohydrates constituting the main part of renewable plant biomass. The research in his group has been supported by grants from several funding agencies, including the Swedish Research Council, the Swedish Energy Agency, Formas, and the Novo Nordisk Foundation.

Johan is a teacher on the courses: (5 HEC; PhD course ), KMB056 – Molecular biotechnology, KBT016 – Biochemistry and biotechnology, KBT185 – Project course in chemistry, and BBT050 – Sustainable food production.

Source: chalmers.se
Image of Johan Larsbrink

Showing 69 publications

2024

Genomic mining of <i>Geobacillus stearothermophilus</i> GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes

M. Carbonaro, M. Aulitto, A. Di Fraia et al
FEBS Open Bio. Vol. 14 (S2), p. 148-149
Other text in scientific journal
2024

Polysaccharide utilization loci from Bacteroidota encode CE15 enzymes with possible roles in cleaving pectin-lignin bonds

Andrea Seveso, Scott Mazurkewich, Sanchari Banerjee et al
Applied and Environmental Microbiology. Vol. 90 (1)
Journal article
2024

Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds

Amanda Sörensen Ristinmaa, Ekaterina Korotkova, Magnus Arntzen et al
Bioresource technology. Vol. 402
Journal article
2024

A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeast Candida intermedia

Kameshwara Venkata Ramana Peri, Le Yuan, Fábio Luis Da Silva Faria Oliveira et al
Applied and Environmental Microbiology. Vol. 90 (10)
Journal article
2024

Exploration of three Dyadobacter fermentans enzymes uncovers molecular activity determinants in CE15

Miriam Carbonaro, Scott Mazurkewich, Gabriella Fiorentino et al
Applied Microbiology and Biotechnology. Vol. 108 (1)
Journal article
2024

Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans

Meng Shu Hao, Scott Mazurkewich, He Li et al
Nature Communications. Vol. 15 (1)
Journal article
2024

Boosting Enzyme Activity in Biomass Conversion by Modulating the Hydrolysis Process of Cellobiohydrolases

Han Liu, Yu Ding, Scott Mazurkewich et al
ACS Catalysis. Vol. 14, p. 16044-16054
Journal article
2024

Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes

Miriam Carbonaro, Martina Aulitto, Scott Mazurkewich et al
New Biotechnology. Vol. 82, p. 14-24
Journal article
2024

Streptomyces castrisilvae sp. nov. and Streptomyces glycanivorans sp. nov., novel soil streptomycetes metabolizing mutan and alternan

Tove Widén, Albert Tafur Rangel, Vincent Lombard et al
International Journal of Systematic and Evolutionary Microbiology. Vol. 74 (9)
Journal article
2023

Regulation of lactose and galactose growth: Insights from a unique metabolic gene cluster in Candida intermedia

Kameshwara Venkata Ramana Peri, Le Yuan, Fábio Luis Da Silva Faria Oliveira et al
Preprint
2023

Filamentous fungi display different behavior during spruce bark degradation.

Amanda Sörensen Ristinmaa, Ekaterina Korotkova, Magnus Artzen et al
Other conference contribution
2023

Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases

Monika Tõlgo, Olav A. Hegnar, Johan Larsbrink et al
Biotechnology for Biofuels and Bioproducts. Vol. 16 (1)
Journal article
2023

Resin acids play key roles in shaping microbial communities during degradation of spruce bark

Amanda Sörensen Ristinmaa, Albert Tafur Rangel, Eduard Kerkhoven et al
Nature Communications. Vol. 14
Journal article
2023

Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans

Scott Mazurkewich, Karoline Scholzen, Rikke Brusch et al
Acta Crystallographica Section D: Structural Biology. Vol. 79, p. 545-555
Journal article
2023

A unique AA5 alcohol oxidase fused with a catalytically inactive CE3 domain from the bacterium Burkholderia pseudomallei

Scott Mazurkewich, Andrea Seveso, Johan Larsbrink
FEBS Letters. Vol. 597 (13), p. 1779-1791
Journal article
2023

Glucuronoyl esterases - enzymes to decouple lignin and carbohydrates and enable better utilization of renewable plant biomass

Johan Larsbrink, L. Lo Leggio
Essays in biochemistry. Vol. 67 (3), p. 493-503
Review article
2023

Filamentous fungi display different behavior during spruce bark degradation.

Amanda Sörensen Ristinmaa, Ekaterina Korotkova, Magnus Artzen et al
Conference poster
2023

Yeasts Have Evolved Divergent Enzyme Strategies To Deconstruct and Metabolize Xylan

Jonas Ravn, Amanda Sörensen Ristinmaa, Tom Coleman et al
Microbiology spectrum. Vol. 11 (3)
Journal article
2022

Characterization of three tannases from Clostridium butyricum active on oak bark extract

Amanda Sörensen Ristinmaa, Johan Larsbrink, Scott Mazurkewich et al
Other conference contribution
2022

Glycan processing in gut microbiomes

Sabina Leanti La Rosa, Matthew Ostrowski, Arturo Vera-Ponce de León et al
Current Opinion in Microbiology. Vol. 67
Review article
2022

Substrate and mechanistic investigation of the tannases CbTan1 and 2 - serine hydrolases active on gallotannins

Tom Coleman, Elise Caron, Amanda Sörensen Ristinmaa et al
Conference poster
2022

Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum

Amanda Sörensen Ristinmaa, Tom Coleman, Leona Cesar et al
Journal of Biological Chemistry. Vol. 298 (4)
Journal article
2022

Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand

Stefan Kittler, Julian Ebner, Mihail Besleaga et al
Journal of Biotechnology. Vol. 359, p. 108-115
Journal article
2022

Enzymes targeting lignin-carbohydrate complexes

Andrea Seveso, Scott Mazurkewich, Johan Larsbrink
Conference poster
2022

Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion

Zhiyou Zong, Scott Mazurkewich, Caroline Pereira et al
Nature Communications. Vol. 13 (1)
Journal article
2022

Alpha vs. Gamma: substrate specificity of CE15 enzymes

Andrea Seveso, Tom Coleman, Kristian B.R.M. Krogh et al
Conference poster
2022

Deciphering key stages, compounds, and microorganisms during microbial degradation of spruce bark

Amanda Sörensen Ristinmaa, Alexander Idström, Phillip B. Pope et al
Conference poster
2021

Performance of Regression Models as a Function of Experiment Noise

Gang Li, Jan Zrimec, Boyang Ji et al
Bioinformatics and Biology Insights. Vol. 15
Journal article
2021

Genomic and transcriptomic analysis of the thermophilic lignocellulose-degrading fungus Thielavia terrestris LPH172

Monika Tõlgo, Silvia Hüttner, Peter Rugbjerg et al
Biotechnology for Biofuels. Vol. 14 (1)
Journal article
2021

The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

Olanrewaju Raji, Jenny Arnling Bååth, Thu V. Vuong et al
FEBS Letters. Vol. 595 (3), p. 351-359
Journal article
2021

Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea cinnamomea

Scott Mazurkewich, Andrea Seveso, Silvia Hüttner et al
Acta Crystallographica Section D: Structural Biology. Vol. 77, p. 1019-1026
Journal article
2021

A polysaccharide utilization locus from the gut bacterium dysgonomonas mossii encodes functionally distinct carbohydrate esterases

Cathleen Kmezik, Scott Mazurkewich, Tomke Meents et al
Journal of Biological Chemistry. Vol. 296
Journal article
2021

Biochemical characterization of three tannases from Clostridium butyricum

Amanda Sörensen Ristinmaa, Tom Coleman, Leona Cesar et al
Conference poster
2021

Polysaccharide degradation by the Bacteroidetes: mechanisms and nomenclature

Lauren S McKee, Sabina Leanti La Rosa, Bjørge Westereng et al
Environmental Microbiology Reports. Vol. 13 (5), p. 559-581
Review article
2021

CAZyme prediction in ascomycetous yeast genomes guides discovery of novel xylanolytic species with diverse capacities for hemicellulose hydrolysis

Jonas Ravn, Martin Engqvist, Johan Larsbrink et al
Biotechnology for Biofuels. Vol. 14 (1)
Journal article
2021

Phylogenetic analysis and in-depth characterization of functionally and structurally diverse CE5 cutinases

Vera Novy, Leonor Vieira Carneiro, Jae Ho Shin et al
Journal of Biological Chemistry. Vol. 297 (5)
Journal article
2021

Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii

Daniel Krska, Scott Mazurkewich, Haley A. Brown et al
Biochemistry. Vol. 60 (27), p. 2206-2220
Journal article
2020

Bacteroidetes bacteria in the soil: Glycan acquisition, enzyme secretion, and gliding motility

Johan Larsbrink, Lauren S McKee
Advances in Applied Microbiology. Vol. 110, p. 63-98
Book chapter
2020

Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae

Scott Mazurkewich, Ronny Helland, Alasdair Mackenzie et al
Scientific Reports. Vol. 10 (1)
Journal article
2020

Multimodular fused acetyl–feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass

Cathleen Kmezik, Cyrielle Bonzom, Lisbeth Olsson et al
Biotechnology for Biofuels. Vol. 13 (1)
Journal article
2019

Structural and biochemical studies of the glucuronoyl esterase OtCE15A illuminate its interaction with lignocellulosic components

Scott Mazurkewich, Jens-Christian N Poulsen, Leila Lo Leggio et al
Journal of Biological Chemistry. Vol. 294 (52), p. 19978-19987
Journal article
2019

Mapping the enzyme machineries of cellulolytic soil-dwelling Bacteroidetes

Marcel Taillefer, Andrea Seveso, Magnus Ø. Arntzen et al
Conference poster
2019

ACTIVE SITE EVOLUTION IN BIOMASS DEGRADING ENZYMES

Kristian E. H. Frandsen, Tobias Tandrup, Jens-Christian N. Poulsen et al
Other conference contribution
2019

Mapping the Enzyme Machineries of Cellulolytic Soil-Dwelling Bacteroidetes

Marcel Taillefer, Andrea Seveso, Magnus Ø. Arntzen et al
Conference poster
2019

Surveying of acid-tolerant thermophilic lignocellulolytic fungi in Vietnam reveals surprisingly high genetic diversity

Vu Nguyen Thanh, Nguyen Thanh Thuy, Han Thi Thu Huong et al
Scientific Reports. Vol. 9 (1)
Journal article
2019

Specific xylan activity revealed for AA9 Lytic Polysaccharide Monooxygenases of the thermophilic fungus Malbranchea cinnamomea by functional characterization

Silvia Hüttner, Anikó Varnai, Dejan Petrović et al
Applied and Environmental Microbiology. Vol. 85 (23)
Journal article
2019

Structure-function analyses reveal that a glucuronoyl esterase from Teredinibacter turnerae interacts with carbohydrates and aromatic compounds

Jenny Arnling Bååth, Scott Mazurkewich, J. C. N. Poulsen et al
Journal of Biological Chemistry. Vol. 294 (16), p. 6635-6644
Journal article
2019

Novel AA9 LPMOs from the thermophilic fungus Thielavia terrestris LPH 172

Monika Tõlgo, Silvia Hüttner, Johan Larsbrink et al
Conference poster
2019

Understanding enzyme-substrate interactions in Carbohydrate Esterase Family 15

Scott Mazurkewich, Jenny Arnling Bååth, Jens-Christian N Poulsen et al
Conference poster
2018

Biochemical and structural features of diverse bacterial glucuronoyl esterases facilitating recalcitrant biomass conversion

Jenny Arnling Bååth, Scott Mazurkewich, Rasmus Meland Knudsen et al
Biotechnology for Biofuels. Vol. 11 (1)
Journal article
2018

Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation

Jenny Arnling Bååth, Antonio Martinez-Abad, Jennie Berglund et al
Biotechnology for Biofuels. Vol. 11 (1)
Journal article
2018

Proteomic Dissection of the Cellulolytic Machineries Used by Soil-Dwelling Bacteroidetes

Marcel Taillefer, Magnus Ø. Arntzen, Bernard Henrissat et al
mSystems. Vol. 3 (6), p. 1-16
Journal article
2018

Biochemical and structural investigation of the CE15 family: Glucuronoyl esterases acting on recalcitrant biomass

Scott Mazurkewich, Jenny Arnling Bååth, Rasmus Meland Knudsen et al
Conference poster
2017

Biochemical and structural diversity in Carbohydrate Esterase Family 15

Scott Mazurkewich, Jenny Arnling Bååth, Amanda Sörensen Ristinmaa et al
Gordon Research Conference "Cellulases and other carbohydrate-active enzymes"
Other conference contribution
2017

Proteomic insights into mannan degradation and protein secretion by the forest floor bacterium Chitinophaga pinensis

Johan Larsbrink, T. R. Tuveng, P. B. Pope et al
Journal of Proteomics. Vol. 156, p. 63-74
Journal article
2017

Proteomic data on enzyme secretion and activity in the bacterium Chitinophaga pinensis

Johan Larsbrink, T. R. Tuveng, Phillip B. Pope et al
Data in Brief. Vol. 11, p. 484-490
Journal article
2017

Combined genome and transcriptome sequencing to investigate the plant cell wall degrading enzyme system in the thermophilic fungus Malbranchea cinnamomea

Silvia Hüttner, T. T. Nguyen, Z. Granchi et al
Biotechnology for Biofuels. Vol. 10 (1)
Journal article
2017

The function of AA9 proteins in the thermophilic fungus Malbranchea cinnamomea

Silvia Hüttner, Kim Anh Dang Thi, Thuy Thanh Nguyen et al
Gordon Research Conference "Cellulases and other carbohydrate-active enzymes"
Conference poster
2016

Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut.

Glyn R Hemsworth, Andrew J Thompson, Judith Stepper et al
Open Biology. Vol. 6 (7)
Journal article
2016

A polysaccharide utilization locus from Flavobacterium johnsoniae enables conversion of recalcitrant chitin

Johan Larsbrink, Yongtao Zhu, Sampada S. Kharade et al
Biotechnology for Biofuels. Vol. 9 (260)
Journal article

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Showing 21 research projects

2024–2029

Discovering biological tools for conversion of bark and its extractives into new products

Johan Larsbrink Industrial Biotechnology
Novo Nordisk Foundation

2024–2026

Application of acidophilic fungi and their enzymes towards valorization of agriculture and forestry biomass

Johan Larsbrink Industrial Biotechnology
Tom Coleman Industrial Biotechnology
Novo Nordisk Foundation

2022–2025

A flexible bacterial consolidated bioprocess for conversion of plant biomass into short-chain fatty acids

Johan Larsbrink Industrial Biotechnology
Verena Siewers Systems and Synthetic Biology
Formas

2022–2024

Towards complete cleavage of lignin-carbohydrate bonds

Johan Larsbrink Industrial Biotechnology
Tom Coleman Industrial Biotechnology
Andrea Seveso Industrial Biotechnology
Merima Hasani Forest Products and Chemical Engineering
Novo Nordisk Foundation

3 publications exist
2022–2022

Circular bioeconomy development via network establishment between Chalmers and Brazilian Biorenewables National Laboratory

Johan Larsbrink Industrial Biotechnology
Lisbeth Olsson Industrial Biotechnology
Carl Johan Franzén Industrial Biotechnology
Verena Siewers Systems and Synthetic Biology
Yvonne Nygård Industrial Biotechnology
Cecilia Geijer Industrial Biotechnology
Formas

2021–2024

Terahertz Technology for Probing Picosecond Protein Dynamics under Physiological Conditions

Helena Rodilla Terahertz and Millimetre Wave Laboratory
Juan Cabello Sánchez Terahertz and Millimetre Wave Laboratory
Scott Mazurkewich Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Swedish Research Council (VR)

2021–2024

Enzymatic deconstruction of microbial polysaccharide networks

Johan Larsbrink Industrial Biotechnology
Lars Evenäs Lars Evenäs Group
Tove Widén Industrial Biotechnology
Swedish Research Council (VR)

2 publications exist
2021–2023

Exploring fungal extremophiles in South East Asian biodiversity as the key for sustainable agriculture and industry

Lisbeth Olsson Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Tom Coleman Industrial Biotechnology
Meera Christopher Industrial Biotechnology
Cao Xuan Bach Biology and Biological Engineering
Swedish Research Council (VR)

2019–2020

Evaluation of the key cellulolytic enzymes from soil-dwelling cellulolytic Bacteroidetes

Johan Larsbrink Industrial Biotechnology
Marcel Taillefer Industrial Biotechnology
ÅForsk

2019–2020

Microbial electro-assisted conversion of biomass to energy carriers

Johan Larsbrink Industrial Biotechnology
Nathan Porter Industrial Biotechnology
Soroush Saheb Alam Industrial Biotechnology
Yvonne Nygård Industrial Biotechnology
Chalmers

2019–2021

Xylanolytic enzymes in yeast

Cecilia Geijer Industrial Biotechnology
Sara Casado López Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Carl Tryggers Stiftelse för Vetenskaplig Forskning

2019–2023

Biochemical conversion of bark

Johan Larsbrink Industrial Biotechnology
Merima Hasani Forest Products and Chemical Engineering
Amanda Sörensen Ristinmaa Industrial Biotechnology
Swedish Energy Agency

9 publications exist
2018–2020

Structure-based engineering of glucuronoyl esterases for separation of lignin and carbohydrates

Johan Larsbrink Industrial Biotechnology
Novo Nordisk Foundation

8 publications exist
2017–2020

CRISPR/Cas9 for the lignocellulose-yeast of the future

Fábio Luis Da Silva Faria Oliveira Industrial Biotechnology
Cecilia Geijer Industrial Biotechnology
Lisbeth Olsson Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Venkat Rao Konasani Industrial Biotechnology
ÅForsk
Swedish Energy Agency
Formas

2017–2021

Development of thermostable enzyme cocktails

Johan Larsbrink Industrial Biotechnology
Daniel Krska Industrial Biotechnology
Formas
Swedish Energy Agency

4 publications exist
2017–2017

Etherases to break covalent bonds between lignin and hemicellulose

Johan Larsbrink Industrial Biotechnology
Swedish Energy Agency

2017–2020

Development of a consolidated bioprocess for conversion of plant biomass to versatile chemical building blocks

Nathan Porter Industrial Biotechnology
Cathleen Kmezik Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Novo Nordisk Foundation
Swedish Research Council (VR)
ÅForsk

5 publications exist
2016–2021

Structure-function studies of enzymes cleaving covalent bonds between lignin and hemicelluloses

Daniel Krska Industrial Biotechnology
Scott Mazurkewich Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Andrea Seveso Industrial Biotechnology
Novo Nordisk Foundation
Interreg

6 publications exist
2016–2018

Mapping of unstudied cellulose-degrading mechanisms in soil bacteria

Johan Larsbrink Industrial Biotechnology
Marcel Taillefer Industrial Biotechnology
Swedish Energy Agency

4 publications exist
2014–2016

Enzymes discovery and production in Vietnam for the production of sustainable biofuels and biochemicals

Lisbeth Olsson Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Silvia Hüttner Industrial Biotechnology
George E Anasontzis Industrial biotechnology
Swedish Research Council (VR)

5 publications exist
2014–2019

Enzymes for selective decomposition of woody biomass

Lisbeth Olsson Industrial Biotechnology
Jenny Arnling Bååth Industrial Biotechnology
Johan Larsbrink Industrial Biotechnology
Knut and Alice Wallenberg Foundation

4 publications exist
There might be more projects where Johan Larsbrink participates, but you have to be logged in as a Chalmers employee to see them.