Mechanism of the glucuronoyl esterase OtCE15A: an alpha/beta hydrolase involved with biomass conversion
Conference poster, 2022

Glucuronoyl esterases are α/β serine hydrolases from Carbohydrate Esterase family 15 (CE15) which cleave an ester linkage that connects lignin to glucuronyl xylan, an important linkage contributing to biomass recalcitrance. Presented here is our recent work on probing the enzyme-substrate interactions of a model glucuronoyl esterase, OtCE15A from the soil bacterium Opitutus terrae, through combined structural, kinetic, and QM/MM investigations. These new investigations greatly add to the knowledge of enzyme-substrate interactions in CE15 and enhances our understanding as to how these enzymes may act in natural conditions, which could aid in industrial biomass conversion technologies.



carbohydrate esterase

glucuronoyl esterase


lignin-carbohydrate complexes.



Scott Mazurkewich

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Zhiyou Zong

Nankai University

Haohao Fu

Nankai University

Wensheng Cai

Nankai University

Xueguang Shao

Nankai University

Caroline Pereira

State University of Campinas

Munir Skaf

State University of Campinas

Leila Lo Leggio

University of Copenhagen

Johan Larsbrink

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

The Microbial Glycobiology Conference
Lisbon, Portugal,

Structure-based engineering of glucuronoyl esterases for separation of lignin and carbohydrates

Novo Nordisk Foundation (27698), 2018-01-01 -- 2020-12-31.

Subject Categories

Biological Sciences

Related datasets

Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion [dataset]

DOI: 10.1038/s41467-022-28938-w URI:

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