Mechanism of the glucuronoyl esterase OtCE15A: an alpha/beta hydrolase involved with biomass conversion
Conference poster, 2022
Glucuronoyl esterases are α/β serine hydrolases from Carbohydrate Esterase family 15 (CE15) which cleave an ester linkage that connects lignin to glucuronyl xylan, an important linkage contributing to biomass recalcitrance. Presented here is our recent work on probing the enzyme-substrate interactions of a model glucuronoyl esterase, OtCE15A from the soil bacterium Opitutus terrae, through combined structural, kinetic, and QM/MM investigations. These new investigations greatly add to the knowledge of enzyme-substrate interactions in CE15 and enhances our understanding as to how these enzymes may act in natural conditions, which could aid in industrial biomass conversion technologies.
carbohydrate esterase
glucuronoxylan
glucuronoxylan
lignin-carbohydrate complexes.
glucuronoyl esterase
CAZy
CE15
Author
Scott Mazurkewich
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Zhiyou Zong
Nankai University
Haohao Fu
Nankai University
Wensheng Cai
Nankai University
Xueguang Shao
Nankai University
Caroline Pereira
State University of Campinas
Munir Skaf
State University of Campinas
Leila Lo Leggio
University of Copenhagen
Johan Larsbrink
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
The Microbial Glycobiology Conference
Lisbon, Portugal,
Lisbon, Portugal,
Structure-based engineering of glucuronoyl esterases for separation of lignin and carbohydrates
Novo Nordisk Foundation (27698), 2018-01-01 -- 2020-12-31.
Subject Categories
Biological Sciences
Related datasets
Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion [dataset]
DOI: 10.1038/s41467-022-28938-w URI: https://www.nature.com/articles/s41467-022-28938-w