Biochemical and structural investigation of the CE15 family: Glucuronoyl esterases acting on recalcitrant biomass
Conference poster, 2018

Glucuronoyl esterases (GEs) are a relatively new class of enzymes which cleave ester linkages between lignin and glucuronoxylan. GEs have been identified in many biomass-degrading microbes and are now classified into the Carbohydrate Esterase Family 15 (CE15). The CE15 family is diverse (as low as 20% sequence identity), however to-date only a few GEs from a small clade of the CE15 phylogenetic tree have been biochemically characterized and only two protein structures have been solved. To investigate the diversity of CE15 members, we have studied a broad range of proteins from across the phylogenetic tree. Enzymes were biochemically characterized and three-dimensional structures for two of the enzymes were solved. Analysis of the structures suggest possible binding sites for lignin fragments and xylooligosaccharides that have not been previously reported. Investigations into the molecular determinants supporting the potential binding sites is being pursued.

crystallography

CE15

glucuronoxylan

lignin

glucuronoyl esterase

biomass

Carbohydrate Esterase

Author

Scott Mazurkewich

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Jenny Arnling Bååth

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Rasmus Meland Knudsen

University of Copenhagen

Jens-Christian N Poulsen

University of Copenhagen

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Leila Lo Leggio

University of Copenhagen

Johan Larsbrink

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

5th Biennial Symposium of Biotechnology Applied to Lignocelluloses
Helsinki , Finland,

Structure-based engineering of glucuronoyl esterases for separation of lignin and carbohydrates

Novo Nordisk Fonden, 2018-01-01 -- 2020-12-31.

Driving Forces

Sustainable development

Subject Categories

Biochemistry and Molecular Biology

Structural Biology

Biocatalysis and Enzyme Technology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Roots

Basic sciences

More information

Latest update

6/19/2019