Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand
Journal article, 2022
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
E
Affinity ligand
Bioreactor
Recombinant production
Protein L
coli
Author
Stefan Kittler
Alfred Gruber GmbH
Vienna University of Technology
Julian Ebner
Vienna University of Technology
Alfred Gruber GmbH
Mihail Besleaga
Vienna University of Technology
Johan Larsbrink
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Barbara Darnhofer
Medical University of Graz
Ruth Birner-Gruenberger
Vienna University of Technology
Medical University of Graz
Silvia Schobesberger
Vienna University of Technology
Christopher K. Akhgar
Vienna University of Technology
Andreas Schwaighofer
Vienna University of Technology
Bernhard Lendl
Vienna University of Technology
Oliver Spadiut
Vienna University of Technology
Journal of Biotechnology
0168-1656 (ISSN) 18734863 (eISSN)
Vol. 359 108-115Driving Forces
Sustainable development
Areas of Advance
Production
Health Engineering
Subject Categories (SSIF 2011)
Biochemistry and Molecular Biology
Medicinal Chemistry
Other Industrial Biotechnology
DOI
10.1016/j.jbiotec.2022.10.002
PubMed
36206851