Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand
Journal article, 2022
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
coli
Affinity ligand
Recombinant production
E
Bioreactor
Protein L
Author
Stefan Kittler
Vienna University of Technology
Alfred Gruber GmbH
Julian Ebner
Vienna University of Technology
Alfred Gruber GmbH
Mihail Besleaga
Vienna University of Technology
Johan Larsbrink
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Barbara Darnhofer
Medical University of Graz
Ruth Birner-Gruenberger
Vienna University of Technology
Medical University of Graz
Silvia Schobesberger
Vienna University of Technology
Christopher K. Akhgar
Vienna University of Technology
Andreas Schwaighofer
Vienna University of Technology
Bernhard Lendl
Vienna University of Technology
Oliver Spadiut
Vienna University of Technology
Journal of Biotechnology
0168-1656 (ISSN) 18734863 (eISSN)
Vol. 359 108-115Subject Categories
Biochemistry and Molecular Biology
Medicinal Chemistry
Other Industrial Biotechnology
DOI
10.1016/j.jbiotec.2022.10.002
PubMed
36206851