Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand
Artikel i vetenskaplig tidskrift, 2022
Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
coli
Affinity ligand
Recombinant production
E
Bioreactor
Protein L
Författare
Stefan Kittler
Technische Universität Wien
Alfred Gruber GmbH
Julian Ebner
Technische Universität Wien
Alfred Gruber GmbH
Mihail Besleaga
Technische Universität Wien
Johan Larsbrink
Chalmers, Biologi och bioteknik, Industriell bioteknik
Barbara Darnhofer
Medizinische Universität Graz
Ruth Birner-Gruenberger
Technische Universität Wien
Medizinische Universität Graz
Silvia Schobesberger
Technische Universität Wien
Christopher K. Akhgar
Technische Universität Wien
Andreas Schwaighofer
Technische Universität Wien
Bernhard Lendl
Technische Universität Wien
Oliver Spadiut
Technische Universität Wien
Journal of Biotechnology
0168-1656 (ISSN) 18734863 (eISSN)
Vol. 359 108-115Ämneskategorier
Biokemi och molekylärbiologi
Läkemedelskemi
Annan industriell bioteknik
DOI
10.1016/j.jbiotec.2022.10.002
PubMed
36206851