The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes
Journal article, 2021

Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

hemicellulases

4-O-methyl d-glucuronic acid

carbohydrate-active enzymes

enzyme adsorption

glucuronoxylan

lignin–carbohydrate complexes

Author

Olanrewaju Raji

University of Toronto

Jenny Arnling Bååth

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Technical University of Denmark (DTU)

Thu V. Vuong

University of Toronto

Johan Larsbrink

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Emma Master

University of Toronto

Aalto University

FEBS Letters

0014-5793 (ISSN)

Vol. 595 3 351-359

Subject Categories

Analytical Chemistry

Bio Materials

Biocatalysis and Enzyme Technology

DOI

10.1002/1873-3468.14019

PubMed

33277689

More information

Latest update

4/16/2021