The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes
Artikel i vetenskaplig tidskrift, 2020

Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

carbohydrate-active enzymes

4-O-methyl d-glucuronic acid

enzyme adsorption

glucuronoxylan

lignin–carbohydrate complexes

hemicellulases

Författare

Olanrewaju Raji

University of Toronto

Jenny Arnling Bååth

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Industriell bioteknik

Thu V. Vuong

University of Toronto

Johan Larsbrink

Chalmers, Biologi och bioteknik, Industriell bioteknik

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Emma Master

University of Toronto

Aalto-Yliopisto

FEBS Letters

0014-5793 (ISSN)

Vol. In Press

Ämneskategorier

Analytisk kemi

Biomaterial

Biokatalys och enzymteknik

DOI

10.1002/1873-3468.14019

PubMed

33277689

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Senast uppdaterat

2021-01-15