Mapping the Enzyme Machineries of Cellulolytic Soil-Dwelling Bacteroidetes
Conference poster, 2019
In order to shed light on the enigmatic cellulolytic systems of these bacteria, we have used quantitative proteomics to map which proteins they produce during growth on cellulose and pectin, respectively, and determined the proteins’ cellular locations. Both bacteria produced similar yet distinct arrays of mostly unstudied putative cellulases during growth, and interestingly, cellulolytic activity was detected not only in the extracellular fraction and outer membrane but also intracellularly. In addition, several glycoside hydrolase family 8 (GH8) enzymes, that have previously been overlooked as potential cellulases in these species, were found to be both abundant and selectively produced during growth on cellulose. These GH8-containing proteins, which comprise large regions of unknown function and range between ~1100-2800 amino acids in total, are currently being functionally characterized to clarify their roles in cellulose turnover.
Author
Marcel Taillefer
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Andrea Seveso
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Magnus Ø. Arntzen
Norwegian University of Life Sciences
Bernard Henrissat
Aix Marseille University
Phillip B. Pope
Norwegian University of Life Sciences
Johan Larsbrink
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Djuronaset, Sweden,
Enzymes for selective decomposition of woody biomass
Knut and Alice Wallenberg Foundation, 2014-01-01 -- 2018-12-31.
Mapping of unstudied cellulose-degrading mechanisms in soil bacteria
Swedish Energy Agency (2015-009561), 2016-01-01 -- 2018-12-31.
Driving Forces
Sustainable development
Subject Categories
Biochemistry and Molecular Biology
Microbiology
Biocatalysis and Enzyme Technology
Areas of Advance
Energy