Mapping the Enzyme Machineries of Cellulolytic Soil-Dwelling Bacteroidetes
Poster (konferens), 2019
In order to shed light on the enigmatic cellulolytic systems of these bacteria, we have used quantitative proteomics to map which proteins they produce during growth on cellulose and pectin, respectively, and determined the proteins’ cellular locations. Both bacteria produced similar yet distinct arrays of mostly unstudied putative cellulases during growth, and interestingly, cellulolytic activity was detected not only in the extracellular fraction and outer membrane but also intracellularly. In addition, several glycoside hydrolase family 8 (GH8) enzymes, that have previously been overlooked as potential cellulases in these species, were found to be both abundant and selectively produced during growth on cellulose. These GH8-containing proteins, which comprise large regions of unknown function and range between ~1100-2800 amino acids in total, are currently being functionally characterized to clarify their roles in cellulose turnover.
Författare
Marcel Taillefer
Chalmers, Biologi och bioteknik, Industriell bioteknik
Andrea Seveso
Chalmers, Biologi och bioteknik, Industriell bioteknik
Magnus Ø. Arntzen
Norges miljø- og biovitenskapelige universitet
Bernard Henrissat
Aix-Marseille Université
Phillip B. Pope
Norges miljø- og biovitenskapelige universitet
Johan Larsbrink
Chalmers, Biologi och bioteknik, Industriell bioteknik
Djuronaset, Sweden,
Enzymer som selektivt bryter bindningar mellan lignin och kolhydrater
Knut och Alice Wallenbergs Stiftelse, 2014-01-01 -- 2018-12-31.
Kartläggning av ostuderade cellulosa-nedbrytningsmekanismer hos jordbakterier
Energimyndigheten (2015-009561), 2016-01-01 -- 2018-12-31.
Drivkrafter
Hållbar utveckling
Ämneskategorier
Biokemi och molekylärbiologi
Mikrobiologi
Biokatalys och enzymteknik
Styrkeområden
Energi