CRISPR/Cas9 for the lignocellulose-yeast of the future
Research Project , 2017 – 2020

A future biobased society relies on the effective conversion of renewable raw materials to fuels, chemicals and materials. For an economically viable production of ethanol from lignocellulose, several process steps need to be optimized. This project aims to develop a new genetic method for integration and optimization of two process bottlenecks: hydrolysis and fermentation of biomass. The method is based on CRISPR/Cas9 technology for efficient, marker free genome editing in industrial xylose fermenting strains of the yeast Saccharomyces cerevisiae. Here, the method is used to create strains that can i) effectively hydrolyze cellulose (the main component of plant biomass) which drastically reduces the need for costly cellulolytic enzymes, and ii) simultaneously ferment cellobiose and xylose for an increased ethanol yield and profitability. In the future, the strains can be developed further into effective sugar platforms for production of advanced biofuels and valuable biochemicals.

Participants

Fábio Luis Da Silva Faria Oliveira (contact)

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Cecilia Geijer

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Venkat Rao Konasani

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Johan Larsbrink

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Funding

Swedish Energy Agency

Project ID: 44742-1
Funding Chalmers participation during 2017–2019

Formas

Project ID: 2017-01417
Funding Chalmers participation during 2018–2020

ÅForsk

Project ID: 17-498
Funding Chalmers participation during 2017

Related Areas of Advance and Infrastructure

Sustainable development

Driving Forces

Energy

Areas of Advance

Life Science Engineering (2010-2018)

Areas of Advance

More information

Latest update

2018-10-16