The role of disaccharides for protein–protein interactions – a SANS study
Journal article, 2019

The disaccharide trehalose has shown outstanding anti-aggregation properties for proteins, which are highly important for the possibility to treat neurodegenerative diseases, such as Alzheimer's and Huntington's disease. However, the role and mechanism of trehalose for such stabilising effects are still largely unknown, partly because a direct structural picture of how trehalose organises around proteins in an aqueous system is missing. Here we compare small-angle neutron scattering (SANS) data on myoglobin in aqueous solutions of either sucrose or trehalose, in order to investigate their effect on protein-protein interactions. We find that both trehalose and sucrose induces a well-defined protein-protein distance, which could explain why these inhibit protein-protein interactions and associated protein aggregation. It does not however explain the superior anti-aggregation effect of trehalose and suggests that the local solvent structures are highly important for explaining the protein stabilisation mechanism. In a broader perspective, these findings are important for understanding the role of sugars in biological stabilisation, and could provide a structural explanation for why trehalose is a promising candidate for the treatment of neurodegenerative and other protein aggregation related diseases.

disaccharides

protein stabilisation

small-angle neutron scattering

Protein aggregation

Author

Christoffer Olsson

Chalmers, Physics, Biological Physics

Jan Swenson

Chalmers, Physics, Biological Physics

Molecular Physics

0026-8976 (ISSN) 1362-3028 (eISSN)

Vol. 117 22 3408-3416

Structure and dynamics of soft and biological materials. I & II

Swedish Research Council (VR), 2016-01-01 -- 2019-12-31.

Swedish Research Council (VR), 2012-01-01 -- 2015-12-31.

Subject Categories

Astronomy, Astrophysics and Cosmology

Atom and Molecular Physics and Optics

Theoretical Chemistry

DOI

10.1080/00268976.2019.1640400

More information

Latest update

3/10/2020