The Liver and Kidneys mediate clearance of cardiac troponin in the rat
Journal article, 2020

Cardiac-specific troponins (cTn), troponin T (cTnT) and troponin I (cTnI) are diagnostic biomarkers when myocardial infarction is suspected. Despite its clinical importance it is still not known how cTn is cleared once it is released from damaged cardiac cells. The aim of this study was to examine the clearance of cTn in the rat. A cTn preparation from pig heart was labeled with fluorescent dye or fluorine 18 (18 F). The accumulation of the fluorescence signal using organ extracts, or the 18 F signal using positron emission tomography (PET) was examined after a tail vein injection. The endocytosis of fluorescently labeled cTn was studied using a mouse hepatoma cell line. Close to 99% of the cTnT and cTnI measured with clinical immunoassays were cleared from the circulation two hours after a tail vein injection. The fluorescence signal from the fluorescently labeled cTn preparation and the radioactivity from the 18F-labeled cTn preparation mainly accumulated in the liver and kidneys. The fluorescently labeled cTn preparation was efficiently endocytosed by mouse hepatoma cells. In conclusion, we find that the liver and the kidneys are responsible for the clearance of cTn from plasma in the rat.

Author

Aida Muslimovic

Sahlgrenska University Hospital

Vincent Fridén

Sahlgrenska University Hospital

Olav Tenstad

University of Bergen

Karin Starnberg

Sahlgrenska University Hospital

Susanne Nyström

Sahlgrenska University Hospital

Emelie Vilhelmsson Wesén

Chalmers, Biology and Biological Engineering, Chemical Biology

Elin Esbjörner Winters

Chalmers, Biology and Biological Engineering, Chemical Biology

Kristoffer Granholm

Sahlgrenska University Hospital

Bertil Lindahl

Akademiska Sjukhuset

Ola Hammarsten

Sahlgrenska University Hospital

Scientific Reports

2045-2322 (ISSN)

Vol. 10 1 6791

Subject Categories

Cell Biology

Cell and Molecular Biology

Pharmacology and Toxicology

DOI

10.1038/s41598-020-63744-8

PubMed

32322013

More information

Latest update

6/3/2020 5