Generic high-capacity protein capture and release by pH control
Journal article, 2020

Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several µg cm-2) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully released with preserved structure and activity.

Author

Gustav Ferrand-Drake Del Castillo

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Andreas Dahlin Group

Rebekah Hailes

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Andreas Dahlin Group

Zeynep Kaya

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Andreas Dahlin Group

Timothy Robson

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry

Andreas Dahlin

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Andreas Dahlin Group

Chemical Communications

1359-7345 (ISSN)

Vol. 56 44 5889-5892

Subject Categories

Analytical Chemistry

DOI

10.1039/d0cc01250e

PubMed

32373823

More information

Latest update

8/4/2020 6