Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
Journal article, 2020

The interaction of exosomes (cell-secreted ∼ 100 nm-sized extracellular vesicles) or membrane-enveloped virions with cellular lipid membranes is often mediated by relatively weak ligand-receptor bonds. Interactions of this type can be studied using vesicles and observing their attachment to receptors located in a lipid bilayer formed at a solid surface. The contact region between a vesicle and the supported lipid bilayer and accordingly the number of ligand-receptor pairs there can be increased by deforming a vesicle. Herein, I (i) estimate theoretically the corresponding deformation energy assuming a disk-like or elongated shape of vesicles, (ii) present the equations allowing one to track such deformations by employing total internal reflection fluorescence microscopy and surface plasmon resonance, and (iii) briefly discuss some related experimental studies.

Multivalent ligand-receptor interaction

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Author

Vladimir Zhdanov

Chalmers, Physics, Biological Physics

Russian Academy of Sciences

European Biophysics Journal

0175-7571 (ISSN) 1432-1017 (eISSN)

Vol. 49 5 395-400

Subject Categories

Physical Chemistry

Cell Biology

Biophysics

DOI

10.1007/s00249-020-01441-0

PubMed

32556429

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Latest update

10/6/2020