Ligand-receptor-mediated attachment of lipid vesicles to a supported lipid bilayer
Artikel i vetenskaplig tidskrift, 2020

The interaction of exosomes (cell-secreted ∼ 100 nm-sized extracellular vesicles) or membrane-enveloped virions with cellular lipid membranes is often mediated by relatively weak ligand-receptor bonds. Interactions of this type can be studied using vesicles and observing their attachment to receptors located in a lipid bilayer formed at a solid surface. The contact region between a vesicle and the supported lipid bilayer and accordingly the number of ligand-receptor pairs there can be increased by deforming a vesicle. Herein, I (i) estimate theoretically the corresponding deformation energy assuming a disk-like or elongated shape of vesicles, (ii) present the equations allowing one to track such deformations by employing total internal reflection fluorescence microscopy and surface plasmon resonance, and (iii) briefly discuss some related experimental studies.

Multivalent ligand-receptor interaction

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Författare

Vladimir Zhdanov

Russian Academy of Sciences

Chalmers, Fysik, Biologisk fysik

European Biophysics Journal

0175-7571 (ISSN) 1432-1017 (eISSN)

Vol. In Press

Ämneskategorier

Fysikalisk kemi

Cellbiologi

Biofysik

DOI

10.1007/s00249-020-01441-0

PubMed

32556429

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Senast uppdaterat

2020-07-09