Evolution from adherent to suspension: systems biology of HEK293 cell line development
Journal article, 2020

The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin.

Author

Magdalena Malm

Royal Institute of Technology (KTH)

Rasool Saghaleyni

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Magnus Lundqvist

Royal Institute of Technology (KTH)

Marco Giudici

Royal Institute of Technology (KTH)

Veronique Chotteau

Royal Institute of Technology (KTH)

Ray Field

AstraZeneca AB

GammaDelta Therapeutics Ltd

Paul G. Varley

AstraZeneca AB

Kymab Limited

Diane Hatton

AstraZeneca AB

Luigi Grassi

AstraZeneca AB

Thomas Svensson

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology, CSBI

Jens B Nielsen

Technical University of Denmark (DTU)

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

J. Rockberg

Royal Institute of Technology (KTH)

Scientific Reports

2045-2322 (ISSN)

Vol. 10 1 18996

Subject Categories

Cell Biology

Cell and Molecular Biology

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

DOI

10.1038/s41598-020-76137-8

PubMed

33149219

More information

Latest update

11/12/2020