Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut
Journal article, 2020

Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. Here, we test the accuracy of AcGGM within the complex endogenous gut microbiome of pigs, wherein we resolve 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially express AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. However, AcGGM-inclusion also manifests a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways occur in neighboring non-mannanolytic populations that produce short-chain fatty acids. Our findings show how intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with potential to create greater modulatory effects at large.

Author

Leszek Michalak

Norwegian University of Life Sciences

John Christian Gaby

Norwegian University of Life Sciences

Leidy Lagos

Norwegian University of Life Sciences

Sabina Leanti La Rosa

Norwegian University of Life Sciences

Torgeir R. Hvidsten

Norwegian University of Life Sciences

Catherine Tétard-Jones

Newcastle University

William G.T. Willats

Newcastle University

Nicolas Terrapon

National Institute of Agronomic Research (INRA)

Aix Marseille University

Vincent Lombard

National Institute of Agronomic Research (INRA)

Aix Marseille University

B. Henrissat

King Abdulaziz University

Aix Marseille University

National Institute of Agronomic Research (INRA)

Johannes Dröge

Chalmers, Mathematical Sciences, Applied Mathematics and Statistics

Magnus Arntzen

Norwegian University of Life Sciences

Live Heldal Hagen

Norwegian University of Life Sciences

Margareth Øverland

Norwegian University of Life Sciences

P. B. Pope

Norwegian University of Life Sciences

Bjørge Westereng

Norwegian University of Life Sciences

Nature Communications

2041-1723 (ISSN)

Vol. 11 1 5773

Subject Categories

Evolutionary Biology

Other Biological Topics

Genetics

DOI

10.1038/s41467-020-19585-0

PubMed

33188211

More information

Latest update

12/1/2020