Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut
Artikel i vetenskaplig tidskrift, 2020
Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. Here, we test the accuracy of AcGGM within the complex endogenous gut microbiome of pigs, wherein we resolve 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially express AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. However, AcGGM-inclusion also manifests a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways occur in neighboring non-mannanolytic populations that produce short-chain fatty acids. Our findings show how intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with potential to create greater modulatory effects at large.
Författare
Leszek Michalak
Norges miljø- og biovitenskapelige universitet
John Christian Gaby
Norges miljø- og biovitenskapelige universitet
Leidy Lagos
Norges miljø- og biovitenskapelige universitet
Sabina Leanti La Rosa
Norges miljø- og biovitenskapelige universitet
Torgeir R. Hvidsten
Norges miljø- og biovitenskapelige universitet
Catherine Tétard-Jones
Newcastle University
William G.T. Willats
Newcastle University
Nicolas Terrapon
Institut national de la recherche agronomique (INRA)
Aix-Marseille Université
Vincent Lombard
Institut national de la recherche agronomique (INRA)
Aix-Marseille Université
B. Henrissat
King Abdulaziz University
Aix-Marseille Université
Institut national de la recherche agronomique (INRA)
Johannes Dröge
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Magnus Arntzen
Norges miljø- og biovitenskapelige universitet
Live Heldal Hagen
Norges miljø- og biovitenskapelige universitet
Margareth Øverland
Norges miljø- og biovitenskapelige universitet
P. B. Pope
Norges miljø- og biovitenskapelige universitet
Bjørge Westereng
Norges miljø- og biovitenskapelige universitet
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 11 1 5773Ämneskategorier
Evolutionsbiologi
Annan biologi
Genetik
DOI
10.1038/s41467-020-19585-0
PubMed
33188211