Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut
Artikel i vetenskaplig tidskrift, 2020

Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have tailored an acetylated galactoglucomannan (AcGGM) fibre to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. Here, we test the accuracy of AcGGM within the complex endogenous gut microbiome of pigs, wherein we resolve 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially express AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. However, AcGGM-inclusion also manifests a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways occur in neighboring non-mannanolytic populations that produce short-chain fatty acids. Our findings show how intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with potential to create greater modulatory effects at large.

Författare

Leszek Michalak

Norges miljø- og biovitenskapelige universitet

John Christian Gaby

Norges miljø- og biovitenskapelige universitet

Leidy Lagos

Norges miljø- og biovitenskapelige universitet

Sabina Leanti La Rosa

Norges miljø- og biovitenskapelige universitet

Torgeir R. Hvidsten

Norges miljø- og biovitenskapelige universitet

Catherine Tétard-Jones

Newcastle University

William G.T. Willats

Newcastle University

Nicolas Terrapon

Institut national de la recherche agronomique (INRA)

Aix-Marseille Université

Vincent Lombard

Institut national de la recherche agronomique (INRA)

Aix-Marseille Université

B. Henrissat

King Abdulaziz University

Aix-Marseille Université

Institut national de la recherche agronomique (INRA)

Johannes Dröge

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Magnus Arntzen

Norges miljø- og biovitenskapelige universitet

Live Heldal Hagen

Norges miljø- og biovitenskapelige universitet

Margareth Øverland

Norges miljø- og biovitenskapelige universitet

P. B. Pope

Norges miljø- og biovitenskapelige universitet

Bjørge Westereng

Norges miljø- og biovitenskapelige universitet

Nature Communications

2041-1723 (ISSN) 20411723 (eISSN)

Vol. 11 1 5773

Ämneskategorier

Evolutionsbiologi

Annan biologi

Genetik

DOI

10.1038/s41467-020-19585-0

PubMed

33188211

Mer information

Senast uppdaterat

2020-12-01