Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires
Journal article, 2021

Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO2-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays.

nanowire sensors

biomarker discovery

antibody microarray

Author

Damiano Verardo

AlignedBio AB

Lund University

Leena Liljedahl

Lund University

Corinna Richter

Lund University

Björn Agnarsson

Chalmers, Physics, Nano and Biophysics

Ulrika Axelsson

Lund University

Christelle N. Prinz

Lund University

Fredrik Höök

Chalmers, Physics, Nano and Biophysics

Carl A. K. Borrebaeck

Lund University

Heiner Linke

Lund University

NANOMATERIALS

2079-4991 (eISSN)

Vol. 11 1 1-11 227

Subject Categories

Medical Laboratory and Measurements Technologies

Analytical Chemistry

Biomedical Laboratory Science/Technology

DOI

10.3390/nano11010227

PubMed

33467141

More information

Latest update

2/19/2021