Fredrik Höök

The Impact of Polyethylene Glycol Lipid Anchors on the Physicochemical Properties, Protein Corona, Function, and Biodistribution of Lipid Nanoparticles

2026

Technological Excellence Requires Human and Social Context

2025

Optical label-free microscopy characterization of dielectric nanoparticles

Developing Endosome Membrane Mimics to Study Lipid Nanocarrier Interactions

2025

Multiparametric functional characterization of individual lipid nanoparticles using surface-sensitive light-scattering microscopy

2025

Comparative Kinetics of Supported Lipid Bilayer Formation on Silica Coated Vertically Oriented Highly Curved Nanowires and Planar Silica Surfaces

Quantitative Detection of Biological Nanoparticles Using Twilight Off-axis Holographic Microscopy: Insights on Complex Formation Between Pegylated Gold Nanoparticles and Lipid Vesicles

2025

Effects of Serum Incubation on Lipid Nanoparticle PEG Shedding, mRNA Retention, and Membrane Interactions

2025

Quantitative Detection of Biological Nanoparticles Using Twilight Off-Axis Holographic Microscopy: Insights on Complex Formation between PEGylated Gold Nanoparticles and Lipid Vesicles

2025

Image analysis optimization for nanowire-based optical detection of molecules

2024

Sub-Nanomolar Detection of Oligonucleotides Using Molecular Beacons Immobilized on Lightguiding Nanowires

2024

Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization

2024

Time-Resolved Inspection of Ionizable Lipid-Facilitated Lipid Nanoparticle Disintegration and Cargo Release at an Early Endosomal Membrane Mimic

2023

Fluorescence excitation enhancement by waveguiding nanowires

2023

Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control

Response to "Letter to the Editor Concerning 'Simultaneous, Single-Particle Measurements of Size and Loading Give Insights into the Structure of Drug-Delivery Nanoparticles'"

Microscopy-based label-free size and refractive index quantification of nanoparticles in unknown media using DAISY

Semiconductor nanowires for biosening

Functionalization of Gold Nanoparticles for Specific Detection of Biological Nanoparticles using Twilight Holographic Microscopy

Temporal monitoring of size, mass, and morphology of weakly interacting systems

The In Vivo Fate of Polycatecholamine Coated Nanoparticles Is Determined by a Fibrinogen Enriched Protein Corona

OPTICAL BIOSENSING AND SINGLE-MOLECULE DETECTION ENHANCED WITH AEROTAXY LIGHTGUIDING NANOWIRES

Supported Lipid Bilayer Platform for Characterization of Lipid Nanoparticle-Based Endosomal Escape Mechanism

Pore formation by melittin in porous silica-supported lipid membranes

2022

Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance

2022

Versailles project on advanced materials and standards (VAMAS) interlaboratory study on measuring the number concentration of colloidal gold nanoparticles

2022

Enhanced Optical Biosensing by Aerotaxy Ga(As)P Nanowire Platforms Suitable for Scalable Production

2022

Probing the Separation Distance between Biological Nanoparticles and Cell Membrane Mimics Using Neutron Reflectometry with Sub-Nanometer Accuracy

2022

Interaction Kinetics of Individual mRNA-Containing Lipid Nanoparticles with an Endosomal Membrane Mimic: Dependence on pH, Protein Corona Formation, and Lipoprotein Depletion

2022

Single-shot self-supervised object detection in microscopy

2022

Time-Resolved Thickness and Shape-Change Quantification using a Dual-Band Nanoplasmonic Ruler with Sub-Nanometer Resolution

2021

Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires

Lipid-Based Bioanalytical Sensors

2021

Control of Polymer Brush Morphology, Rheology, and Protein Repulsion by Hydrogen Bond Complexation

2021

A vaccine combination of lipid nanoparticles and a cholera toxin adjuvant derivative greatly improves lung protection against influenza virus infection

2021

Physicochemical tools for studying virus interactions with targeted cell membranes in a molecular and spatiotemporally resolved context

2021

Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles

2021

Fast and Accurate Nanoparticle Characterization Using Deep-Learning-Enhanced Off-Axis Holography

2021

Investigation of Self-Emulsifying Drug-Delivery System Interaction with a Biomimetic Membrane under Conditions Relevant to the Small Intestine

2021

Diffusion of Lipid Nanovesicles Bound to a Lipid Membrane Is Associated with the Partial-Slip Boundary Condition

Simultaneous, Single-Particle Measurements of Size and Loading Give Insights into the Structure of Drug-Delivery Nanoparticles

2021

Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates

2021

Determination of Nanosized Adsorbate Mass in Solution Using Mechanical Resonators: Elimination of the so Far Inseparable Liquid Contribution

Fluorescence excitation enhancement in nanowires for biosensing

2021

TIRF Microscopy-Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica

Size and Refractive Index Determination of Subwavelength Particles and Air Bubbles by Holographic Nanoparticle Tracking Analysis

A Nanofluidic Device for Multiplexed Analysis of Single Exosomes

Independent Size and Fluorescence Emission Determination of Individual Biological Nanoparticles Reveals that Lipophilic Dye Incorporation Does Not Scale with Particle Size

2020

Single-vesicle imaging reveals lipid-selective and stepwise membrane disruption by monomeric α-synuclein

2020

Enhancing the cellular uptake and antibacterial activity of rifampicin through encapsulation in mesoporous silica nanoparticles

2020

Influence of Bile Composition on Membrane Incorporation of Transient Permeability Enhancers

Single-molecule biosensors: Recent advances and applications

High throughput size-determination and multiplexed fluorescence analysis of single biological particles in a nanofluidic device

Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators

Charged Polystyrene Nanoparticles Near a SiO2/Water Interface

2019

Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells

Gel Phase 1,2-Distearoyl- sn-glycero-3-phosphocholine-Based Liposomes Are Superior to Fluid Phase Liposomes at Augmenting Both Antigen Presentation on Major Histocompatibility Complex Class II and Costimulatory Molecule Display by Dendritic Cells in Vitro

Quantitative Detection of Biological Nanoparticles in Solution via Their Mediation of Colocalization of Fluorescent Liposomes

Single-Molecule Detection with Lightguiding Nanowires: Determination of Protein Concentration and Diffusivity in Supported Lipid Bilayers

Competition for membrane receptors: Norovirus detachment via lectin attachment

Avidity-Based Affinity Enhancement Using Nanoliposome-Amplified SPR Sensing Enables Low Picomolar Detection of Biologically Active Neuregulin 1

Effective Refractive Index and Lipid Content of Extracellular Vesicles Revealed Using Optical Waveguide Scattering and Fluorescence Microscopy

2018

Sticking particles to solid surfaces using Moringa oleifera proteins as a glue

2018

MicroRNA Detection by DNA-Mediated Liposome Fusion

Antenna-Enhanced Fluorescence Correlation Spectroscopy Resolves Calcium-Mediated Lipid-Lipid Interactions

2018

Bioinspired, nanoscale approaches in contemporary bioanalytics (Review)

2018

Low-temperature fabrication and characterization of a symmetric hybrid organic–inorganic slab waveguide for evanescent light microscopy

Membrane Deformation Induces Clustering of Norovirus Bound to Glycosphingolipids in a Supported Cell-Membrane Mimic

2018

Waveguide structure

2018

Toward multiplexed quantification of biomolecules on surfaces using time-of-flight secondary ion mass spectrometry

2018

Nanomaterial interactions with biomembranes: Bridging the gap between soft matter models and biological context

2018

Pentacyclic adenine: A versatile and exceptionally bright fluorescent DNA base analogue

Affinity Purification and Single-Molecule Analysis of Integral Membrane Proteins from Crude Cell-Membrane Preparations

Structure and Composition of Native Membrane Derived Polymer-Supported Lipid Bilayers

2018

Nanometer-scale molecular organization in lipid membranes studied by time-of-flight secondary ion mass spectrometry

Spatiotemporal Kinetics of Supported Lipid Bilayer Formation on Glass via Vesicle Adsorption and Rupture

Single Proteoliposomes with E. coli Quinol Oxidase: Proton Pumping without Transmembrane Leaks

Nanopartiklar kan förbättra avbildningsteknik och diagnostik [Nanoparticles can improve imaging and diagnostics]

2017

Detachment of Membrane Bound Virions by Competitive Ligand Binding Induced Receptor Depletion

Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films

Histo-Blood Group Antigen Presentation Is Critical for Binding of Norovirus VLP to Glycosphingolipids in Model Membranes

Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles

2016

Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity

2016

Mucosal Vaccine Development Based on Liposome Technology

Histo-blood group antigen presentation is critical for norovirus VLP binding to glycosphingolipids in membranes

Kinetics of enzyme-mediated hydrolysis of lipid vesicles

Monitoring of single and double lipid membrane formation with high spatiotemporal resolution using evanescent light scattering microscopy

Quantification of Multivalent Interactions by Tracking Single Biological Nanoparticle Mobility on a Lipid Membrane

Supported lipid bilayer repair mediated by AH peptide

Nonspecific Colloidal-Type Interaction Explains Size-Dependent Specific Binding of Membrane-Targeted Nanoparticles

2016

Affinity Capturing and Surface Enrichment of a Membrane Protein Embedded in a Continuous Supported Lipid Bilayer

Hydrodynamic Propulsion of Liposomes Electrostatically Attracted to a Lipid Membrane Reveals Size-Dependent Conformational Changes

Imaging of Amyloid-β in Alzheimer’s disease transgenic mouse brains with Time-of-Flight Secondary Ion Mass Spectrometry using Immunoliposomes

Influence of the Evanescent Field Decay Length on the Sensitivity of Plasmonic Nanodisks and Nanoholes

Diffusion-limited attachment of large spherical particles to flexible membrane-immobilized receptors

Assembly of RNA nanostructures on supported lipid bilayers

2015

Total internal reflection fluorescence microscopy for determination of size of individual immobilized vesicles: Theory and experiment

Evanescent Light-Scattering Microscopy for Label-Free Interfacial Imaging: From Single Sub-100 nm Vesicles to Live Cells

2015

Design of Surface Modifications for Nanoscale Sensor Applications

Interaction of Virus-Like Particles with Vesicles Containing Glycolipids: Kinetics of Detachment

Hydrolysis of a Lipid Membrane by Single Enzyme Molecules: Accurate Determination of Kinetic Parameters.

Kinetics of enzymatic reactions in lipid membranes containing domains

Equilibrium-Fluctuation Analysis for Interaction Studies between Natural Ligands and Single G Protein-Coupled Receptors in Native Lipid Vesicles

Drug Discovery at the Single Molecule Level: Inhibition-in-Solution Assay of Membrane-Reconstituted beta-Secretase Using Single-Molecule Imaging

Neutralized Chimeric Avidin Binding at a Reference Biosensor Surface

Preserved Transmembrane Protein Mobility in Polymer-Supported Lipid Bilayers Derived from Cell Membranes

2015

Strongly Stretched Protein Resistant Poly(ethylene glycol) Brushes Prepared by Grafting-To

Optical waveguide-based single-molecule studies for medical diagnostics and drug screening applications

Simultaneous imaging of amyloid-β and lipids in brain tissue using antibody-coupled liposomes and time-of-flight secondary ion mass spectrometry

Plasmon-enhanced four-wave mixing by nanoholes in thin gold films

Liposome binding for multiplexed biomolecule detection and imaging using ToF-SIMS

Label-Free Measurements of the Diffusivity of Molecules in Lipid Membranes

Plasmonic Nanopores in Metal-Insulator-Metal Films

Optical waveguide-based single-molecule studies in diagnostics and drug screening

Determination of exosome concentration in solution using surface plasmon resonance spectroscopy.

2013

Equilibrium-fluctuation-analysis of single liposome binding events reveals how cholesterol and Ca2+ modulate glycosphingolipid trans-interactions

2013

Nucleation in mesoscopic systems under transient conditions: Peptide-induced pore formation in vesicles

Single lipid vesicle assay for characterizing single-enzyme kinetics of phospholipid hydrolysis in a complex biological fluid.

2013

A virus biosensor with single virus-particle sensitivity based on fluorescent vesicle labels and equilibrium fluctuation analysis

Hydrodynamic separation of proteins in supported lipid bilayers confined by gold barriers

Promises and challenges of nanoplasmonic devices for refractometric biosensing

A Secretory Artificial Cell for Exocytosis

Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes

Nanoplasmonic sensing combined with artificial cell membranes

Interaction of virions with membrane glycolipids

Material-Selective Surface Chemistry for Nanoplasmonic Sensors: Optimizing Sensitivity and Controlling Binding to Local Hot Spots

Single Vesicle Analysis Reveals Nanoscale Membrane Curvature Selective Pore Formation in Lipid Membranes by an Antiviral α-Helical Peptide

Time-Resolved Surface-Enhanced Ellipsometric Contrast Imaging for Label-Free Analysis of Biomolecular Recognition Reactions on Glycolipid Domains

2012

Norovirus GII.4 Virus-like Particles Recognize Galactosylceramides in Domains of Planar Supported Lipid Bilayers.

Design of Intelligent Surface Modifications and Optimal Liquid Handling for Nanoscale Bioanalytical Sensors

Formation and Diffusivity Characterization of Supported Lipid Bilayers with Complex Lipid Compositions

Kinetics of the enzyme-vesicle interaction including the formation of rafts and membrane strain

High throughput fabrication of plasmonic nanostructures in nanofluidic pores for biosensing applications

2012

A functioning artificial secretory cell

2011

Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids

Phase transitions in adsorbed lipid vesicles measured using a quartz crystal microbalance with dissipation monitoring

Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces

Continuous Lipid Bilayers Derived from Cell Membranes for Spatial Molecular Manipulation

Resonance-Mode Electrochemical Impedance Measurements of Silicon Dioxide Supported Lipid Bilayer Formation and Ion Channel Mediated Charge Transport

Kinetics of Ligand Binding to Membrane Receptors from Equilibrium Fluctuation Analysis of Single Binding Events

Effects of Surface Pressure and Internal Friction on the Dynamics of Shear-Driven Supported Lipid Bilayers

Heavy Meromyosin Molecules Extending More Than 50 nm above Adsorbing Electronegative Surfaces

Investigation of Adsorption and Cross-Linking of a Mussel Adhesive Protein Using Attenuated Total Internal Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR)

Nanoplasmonic biosensing with on-chip electrical detection

DNA Binding to Zwitterionic Model Membranes

Probing molecular recognition on the single molecule level

A simple nanoplasmonic sensor with microliter sample handling

RNA and DNA interactions with zwitterionic and charged lipid membranes - A DSC and QCM-D study

Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates

Locally Functionalized Short-Range Ordered Nanoplasmonic Pores for Bioanalytical Sensing

Detecting water transport through lipid membranes on a millisecond level using microfluidics and fluorescent dyes

Spatial-Resolution Limits in Mass Spectrometry Imaging of Supported Lipid Bilayers and Individual Lipid Vesicles

Shear-driven motion of supported lipid bilayers in microfluidic channels

Molecular motors on lipid bilayers and silicon dioxide: different driving forces for adsorption

Simulation of dissociation of DNA duplexes attached to the surface

Sealing of Submicrometer Wells by a Shear-Driven Lipid Bilayer

Refractive-Index-Based Screening of Membrane-Protein-Mediated Transfer across Biological Membranes

High-Performance Biosensing Using Arrays of Plasmonic Nanotubes

Liposome-Based Chemical Barcodes for Single Molecule DNA Detection Using Imaging Mass Spectrometry

Separation of Bulk Effects and Bound Mass during Adsorption of Surfactants Probed by Quartz Crystal Microbalance with Dissipation: Insight into Data Interpretation

Improving the Limit of Detection of Nanoscale Sensors by Directed Binding to High-Sensitivity Areas

Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

QCM-D studies of human norovirus VLPs binding to glycosphingolipids in supported lipid bilayers reveal strain-specific characteristics.

Mechanical Behavior of a Supported Lipid Bilayer under External Shear Forces

Alpha-Helical Peptide-Induced Vesicle Rupture Revealing New Insight into the Vesicle Fusion Process As Monitored in Situ by Quartz Crystal Microbalance-Dissipation and Reflectometry

High-resolution microspectroscopy pf plasmonic nanostructures for miniaturized biosensing

Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide

Shear-Driven Motion of Supported Lipid Bilayers in Microfluidic Channels

Self-assembly formation of multiple DNA-tethered lipid bilayers

Kinetic and thermodynamic characterization of single-mismatch discrimination using single-molecule imaging

Quantitative Biological Surface Science: Challenges and Recent Advances

Addressable adsorption of lipid vesicles and subsequent protein interaction studies

A combined reflectometry and quartz crystal microbalance with dissipation setup for surface interaction studies

Nanoplasmonic biosensing with focus on short-range ordered nanoholes in thin metal films

Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing

Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

Controlled radial distribution of nanoscale vesicles during binding to an oscillating QCM surface

Characterization of a proton pumping transmembrane protein incorporated into a supported three-dimensional matrix of proteoliposomes

Investigation of binding event perturbations caused by elevated QCM-D oscillation amplitude

A multitechnique study of liposome adsorption an Au and I ipid bilayer formation on SiO2

Suppression of binding events via external perturbation with emphasis on QCM

Light-Regulated release of liposomes from phospholipid membranes via photoresponsive polymer-DNA conjugates

Quantification of Oligonucleotide Modifications of Small Unilamellar Lipid Vesicles

Plasmonic Sensing Characteristics o f Single Nanometric Holes

Gravimetric antigen detection utilizing anitbody-modified lipid bilayers

Light-activated desorption of photoactive polyelectrolytes from supported lipid bilayers

Localized surface plasmon sensing of lipid-membrane-mediated biorecognition events

Lateral mobility of tethered vesicle - DNA assemblies

A dual-frequency QCM-D setup operating at elevated oscillation amplitudes

Utilizing adsorbed proteoliposomes trapped in a non-ruptured state on SiO2 for amplified detection of membrane proteins

Micropatterening of DNA-tagged visicles

Bivalent Cholesterol-based coupling of oligonucleotides to lipid membrane assemblies

Supported lipid bilayer based surface modifications - An approach to control cell attachment and cell differentiation

Simultaneous Surface Plasmon Resonance and Quartz Crystal Microbalance with Dissipation Monitoring Measurements of Biomolecular Adsorption Events Involving Structural Transformations and Variations in Coupled Water

DNA-based formation of a supported, three-dimensional lipid vesicle matrix probed by QCM-D and SPR

The use of quartz crystal microbalance with dissipation for the measurement of protein-protein interactions: a qualitative and quantitative analysis of the interactions between molecular chaperones

Formation of Supported Lipid Bilayer Membranes on SiO2 from Proteoliposomes Containing Transmembrane Proteins

In situ peptide-modified supported lipid bilayers for controlled cell attachment

A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation

Characterization of PNA and DNA Immobilization and Subsequent Hybridization with DNA Using Acoustic-Shear-Wave Attenuation Measurements

Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: a quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study

Surface preparation and evaluation for biointerfaces

Measurement of the Dynamics of Exocytosis and Vesicle Retrieval at Cell Populations using a Quartz Crystal Microbalance

Formation of protein-containing supported lipid bilayers on SiO2

Functional surfaces for biorecognition and sensing

Adsorption behavior and enzymatically or chemically induced cross-linking of a mussel adhesive protein

Development of a Novel QCM Technique for Protein Adsorption Studies

Protein adsorption with a newly developed quartz crystal microbalance by Fredrik Höök