Sealing of Submicrometer Wells by a Shear-Driven Lipid Bilayer
Journal article, 2010
A supported lipid bilayer (SLB) was formed in a microfluidic channel by vesicle fusion. The SLB, formed on a flat part of the surface, was driven by the shear forces of a bulk flow above the SLB to a part of the surface with embedded submicrometer wells. When using a bulk solution with a pH of 9.5 the advancing lipid bilayer sealed the wells, creating free-spanning membranes, whereas at a pH of 8.0 the SLB instead followed the contour of the wells.
microfluidics
SILICON
CHANNELS
molecule encapsulation
MONOLAYERS
SUPPORTED PHOSPHOLIPID-BILAYERS
MEMBRANES
KINETICS
Spreading lipid bilayers
HYDROPHILIC SURFACES
BIOSENSOR
NANOPORE ARRAYS
DIFFUSION
nanostructures
black lipid membranes
Author
Peter Jönsson
Chalmers, Applied Physics, Biological Physics
Magnus Jonsson
Chalmers, Applied Physics, Biological Physics
Fredrik Höök
Chalmers, Applied Physics, Biological Physics
Nano Letters
1530-6984 (ISSN) 1530-6992 (eISSN)
Vol. 10 5 1900-1906Subject Categories (SSIF 2011)
Physical Sciences
DOI
10.1021/nl100779k