Sealing of Submicrometer Wells by a Shear-Driven Lipid Bilayer
Artikel i vetenskaplig tidskrift, 2010
A supported lipid bilayer (SLB) was formed in a microfluidic channel by vesicle fusion. The SLB, formed on a flat part of the surface, was driven by the shear forces of a bulk flow above the SLB to a part of the surface with embedded submicrometer wells. When using a bulk solution with a pH of 9.5 the advancing lipid bilayer sealed the wells, creating free-spanning membranes, whereas at a pH of 8.0 the SLB instead followed the contour of the wells.
microfluidics
SILICON
CHANNELS
molecule encapsulation
MONOLAYERS
SUPPORTED PHOSPHOLIPID-BILAYERS
MEMBRANES
KINETICS
Spreading lipid bilayers
HYDROPHILIC SURFACES
BIOSENSOR
NANOPORE ARRAYS
DIFFUSION
nanostructures
black lipid membranes
Författare
Peter Jönsson
Chalmers, Teknisk fysik, Biologisk fysik
Magnus Jonsson
Chalmers, Teknisk fysik, Biologisk fysik
Fredrik Höök
Chalmers, Teknisk fysik, Biologisk fysik
Nano Letters
1530-6984 (ISSN) 1530-6992 (eISSN)
Vol. 10 5 1900-1906Ämneskategorier
Fysik
DOI
10.1021/nl100779k