Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates
Journal article, 2021

Supported lipid bilayers (SLBs) serve important roles as minimalistic models of cellular membranes in multiple diagnostic and pharmaceutical applications as well as in the strive to gain fundamental insights about their complex biological function. To further expand the utility of SLBs, there is a need to go beyond simple lipid compositions to thereby better mimic the complexity of native cell membranes, while simultaneously retaining their compatibility with a versatile range of analytical platforms. To meet this demand, we have in this work explored SLB formation on PEDOT:PSS/silica nanoparticle composite films and mesoporous silica films, both capable of transporting ions to an underlying conducting PEDOT:PSS film. The SLB formation process was evaluated by using the quartz crystal microbalance with dissipation (QCM-D) monitoring, total internal reflection fluorescence (TIRF) microscopy, and fluorescence recovery after photobleaching (FRAP) for membranes made of pure synthetic lipids with or without the reconstituted membrane protein β-secretase 1 (BACE1) as well as cell-derived native lipid vesicles containing overexpressed BACE1. The mesoporous silica thin film was superior to the PEDOT:PSS/silica nanoparticle composite, providing successful formation of bilayers with high lateral mobility and low defect density even for the most complex native cell membranes.

Author

Hanna Gustafsson

Chalmers, Physics, Biological Physics

Josefin Nissa

Linköping University

Hudson Pace

Chalmers, Physics, Biological Physics

Olov Wahlsten

Chalmers, Physics, Biological Physics

Anders Gunnarsson

AstraZeneca AB

Daniel T. Simon

Linköping University

M. Berggren

Linköping University

Fredrik Höök

Chalmers, Physics, Nano and Biophysics

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 37 18 5494-5505

Subject Categories

Physical Chemistry

Cell Biology

Biophysics

DOI

10.1021/acs.langmuir.1c00175

PubMed

33929845

More information

Latest update

6/11/2021