Interaction of virions with membrane glycolipids
Journal article, 2012

Cellular membranes contain various lipids including glycolipids (GLs). The hydrophilic head groups of GLs extend from the membrane into the aqueous environment outside the cell where they act as recognition sites for specific interactions. The first steps of interaction of virions with cells often include contacts with GLs. To clarify the details of such contacts, we have used the total internal reflection fluorescence microscopy to explore the interaction of individual unlabelled virus-like particles (or, more specifically, norovirus protein capsids), which are firmly bound to a lipid bilayer, and fluorescent vesicles containing glycosphingolipids (these lipids form a subclass of GLs). The corresponding binding kinetics were earlier found to be kinetically limited, while the detachment kinetics were logarithmic over a wide range of time. Here, the detachment rate is observed to dramatically decrease with increasing concentration of glycosphingolipids from 1% to 8%. This effect has been analytically explained by using a generic model describing the statistics of bonds in the contact area between a virion and a lipid membrane. Among other factors, the model takes the formation of GL domains into account. Our analysis indicates that in the system under consideration, such domains, if present, have a characteristic size smaller than the contact area between the vesicle and the virus-like particle.

monte-carlo

viruses

single virus

infection pathway

enveloped

entry

lipid rafts

intracellular viral kinetics

model

cell

norwalk virus

Author

Marta Bally

Chalmers, Applied Physics, Biological Physics

Kristian Dimitrievski

Chalmers, Applied Physics, Chemical Physics

Göran Larson

University of Gothenburg

Vladimir Zhdanov

Chalmers, Applied Physics, Chemical Physics

Fredrik Höök

Chalmers, Applied Physics, Biological Physics

Physical Biology

1478-3967 (ISSN) 1478-3975 (eISSN)

Vol. 9 2 Article Number: 026011-

Subject Categories

Physical Chemistry

Infectious Medicine

Clinical Laboratory Medicine

Microbiology in the medical area

DOI

10.1088/1478-3975/9/2/026011

More information

Created

10/6/2017