Interaction of virions with membrane glycolipids
Artikel i vetenskaplig tidskrift, 2012

Cellular membranes contain various lipids including glycolipids (GLs). The hydrophilic head groups of GLs extend from the membrane into the aqueous environment outside the cell where they act as recognition sites for specific interactions. The first steps of interaction of virions with cells often include contacts with GLs. To clarify the details of such contacts, we have used the total internal reflection fluorescence microscopy to explore the interaction of individual unlabelled virus-like particles (or, more specifically, norovirus protein capsids), which are firmly bound to a lipid bilayer, and fluorescent vesicles containing glycosphingolipids (these lipids form a subclass of GLs). The corresponding binding kinetics were earlier found to be kinetically limited, while the detachment kinetics were logarithmic over a wide range of time. Here, the detachment rate is observed to dramatically decrease with increasing concentration of glycosphingolipids from 1% to 8%. This effect has been analytically explained by using a generic model describing the statistics of bonds in the contact area between a virion and a lipid membrane. Among other factors, the model takes the formation of GL domains into account. Our analysis indicates that in the system under consideration, such domains, if present, have a characteristic size smaller than the contact area between the vesicle and the virus-like particle.

monte-carlo

viruses

single virus

infection pathway

enveloped

entry

lipid rafts

intracellular viral kinetics

model

cell

norwalk virus

Författare

Marta Bally

Chalmers, Teknisk fysik, Biologisk fysik

Kristian Dimitrievski

Chalmers, Teknisk fysik, Kemisk fysik

Göran Larson

Göteborgs universitet

Vladimir Zhdanov

Chalmers, Teknisk fysik, Kemisk fysik

Fredrik Höök

Chalmers, Teknisk fysik, Biologisk fysik

Physical Biology

1478-3967 (ISSN) 1478-3975 (eISSN)

Vol. 9 2 Article Number: 026011-

Ämneskategorier

Fysikalisk kemi

Infektionsmedicin

Klinisk laboratoriemedicin

Mikrobiologi inom det medicinska området

DOI

10.1088/1478-3975/9/2/026011

Mer information

Skapat

2017-10-06