MicroRNA Detection by DNA-Mediated Liposome Fusion
Journal article, 2018

Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal-generating molecules. In this work, sequence-specific DNA-mediated liposome fusion is used for the highly selective detection of microRNA. The detection of miR-29a, a known flu biomarker, is demonstrated down to 18 nm within 30 min with high specificity by using a standard laboratory microplate reader. Furthermore, one order of magnitude improvement in the limit of detection is demonstrated by using a novel imaging technique combined with an intensity fluctuation analysis, which is coined two-color fluorescence correlation microscopy.

biosensors

nucleic acids

liposomes

membrane fusion

FRET

Author

Coline Jumeaux

Imperial College London

Olov Wahlsten

Chalmers, Physics, Biological Physics

Stephan Block

Freie Universität Berlin

Chalmers, Physics, Biological Physics

Eunjung Kim

Imperial College London

Rona Chandrawati

Imperial College London

The University of Sydney

Philip D. Howes

Imperial College London

Fredrik Höök

Chalmers, Physics, Biological Physics

Molly M. Stevens

Imperial College London

ChemBioChem

1439-4227 (ISSN) 1439-7633 (eISSN)

Vol. 19 5 434-438

Surface-sensitive microscopy for studies of cellular membranes

Swedish Research Council (VR), 2015-01-01 -- 2018-12-31.

Subject Categories

Biochemistry and Molecular Biology

Analytical Chemistry

Biophysics

DOI

10.1002/cbic.201700592

More information

Latest update

3/22/2019