High throughput size-determination and multiplexed fluorescence analysis of single biological particles in a nanofluidic device
Paper in proceedings, 2019

Biological nanoparticles, such as exosomes and viruses, are responsible for a multitude of important functions, but methods to characterize them on the single particle level are rare. We here present a nanofluidic platform for multi-parametric characterization of biological nanoparticles with high throughput. The device consists of feeding microchannels and an array of ~100 nanochannels where the nanoparticles can be characterized. We determine the size by analyzing the Brownian motion of the particles and quantify their content based on fluorescence imaging of up to three different colors. We successfully benchmark our method against existing techniques, such as Nanoparticle Tracking Analysis (NTA).

Nanofluidics

Fluorescence microscopy

Biological nanoparticles

Author

Quentin Lubart

Chalmers, Biology and Biological Engineering, Chemical Biology

Sune Levin

Chalmers, Biology and Biological Engineering, Chemical Biology

Stephan Block

Freie Universität Berlin

Silver Jõemetsa

Chalmers, Physics, Biological Physics

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Fredrik Höök

Chalmers, Physics

Marta Bally

Umeå University

Elin Esbjörner Winters

Chalmers, Biology and Biological Engineering, Chemical Biology

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

1420-1421

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Subject Categories

Materials Chemistry

Other Chemistry Topics

Water Treatment

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Latest update

11/13/2020