High throughput size-determination and multiplexed fluorescence analysis of single biological particles in a nanofluidic device
Paper i proceeding, 2019

Biological nanoparticles, such as exosomes and viruses, are responsible for a multitude of important functions, but methods to characterize them on the single particle level are rare. We here present a nanofluidic platform for multi-parametric characterization of biological nanoparticles with high throughput. The device consists of feeding microchannels and an array of ~100 nanochannels where the nanoparticles can be characterized. We determine the size by analyzing the Brownian motion of the particles and quantify their content based on fluorescence imaging of up to three different colors. We successfully benchmark our method against existing techniques, such as Nanoparticle Tracking Analysis (NTA).

Nanofluidics

Fluorescence microscopy

Biological nanoparticles

Författare

Quentin Lubart

Chalmers, Biologi och bioteknik, Kemisk biologi

Sune Levin

Chalmers, Biologi och bioteknik, Kemisk biologi

Stephan Block

Freie Universität Berlin

Silver Jõemetsa

Chalmers, Fysik, Biologisk fysik

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Fredrik Höök

Chalmers, Fysik

Marta Bally

Umeå universitet

Elin Esbjörner Winters

Chalmers, Biologi och bioteknik, Kemisk biologi

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

1420-1421

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Basel, Switzerland,

Ämneskategorier

Materialkemi

Annan kemi

Vattenbehandling

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Senast uppdaterat

2020-11-13