Versailles project on advanced materials and standards (VAMAS) interlaboratory study on measuring the number concentration of colloidal gold nanoparticles

Caterina Minelli; Magdalena Wywijas; Dorota Bartczak; Susana Cuello-Nunez; Heidi Goenaga Infante; Jerome Deumer; Christian Gollwitzer; Michael Krumrey; Karen E. Murphy; Monique E. Johnson; Antonio R. Montoro Bustos; Ingo H. Strenge; Bertrand Faure; Peter Hoghoj; Vivian Tong; Loic Burr; Karin Norling; Fredrik Höök; Matthias Roesslein; Jovana Kocic; Lyndsey Hendriks; Vikram Kestens; Yannic Ramaye; Maria C. Contreras Lopez; Guy Auclair; Dora Mehn; Douglas Gilliland; Annegret Potthoff; Kathrin Oelschlagel; Jutta Tentschert; Harald Jungnickel; Benjamin C. Krause; Yves U. Hachenberger; Philipp Reichardt; Andreas Luch; Thomas E. Whittaker; Molly M. Stevens; Shalini Gupta; Akash Singh; Fang-hsin Lin; Yi-Hung Liu; Anna Luisa Costa; Carlo Baldisserri; Rid Jawad; Samir E. L. Andaloussi; Margaret N. Holme; Tae Geol Lee; Minjeong Kwak; Jaeseok Kim; Johanna Ziebel; Cedric Guignard; Sebastien Cambier; Servane Contal; Arno C. Gutleb; Jan Kuba Tatarkiewicz; Bartlomiej J. Jankiewicz; Bartosz Bartosewicz; Xiaochun Wu; Jeffrey A. Fagan; Elisabeth Elje; Elise Runden-Pran; Maria Dusinska; Inder Preet Kaur; David Price; Ian Nesbitt; Sarah O'Reilly; Ruud J. B. Peters; Guillaume Bucher; Dennis Coleman; Angela J. Harrison; Antoine Ghanem; Anne Gering; Eileen McCarron; Niamh Fitzgerald; Geert Cornelis; Jani Tuoriniemi; Midori Sakai; Hidehisa Tsuchida; Ciaran Maguire; Adriele Prina-Mello; Alan J. Lawlor; Jessica Adams; Carolin L. Schultz; Doru Constantin; Nguyen Thi Kim Thanh; Le Duc Tung; Luca Panariello; Spyridon Damilos; Asterios Gavriilidis; Iseult Lynch; Benjamin Fryer; Ana Carrazco Quevedo; Emily Guggenheim; Sophie Briffa; Eugenia Valsami-Jones; Yuxiong Huang; Arturo A. Keller; Virva-Tuuli Kinnunen; Siiri Peramaki; Zeljka Krpetic; Michael Greenwood; Alexander G. Shard

doi:10.1039/d1nr07775a

Versailles project on advanced materials and standards (VAMAS) interlaboratory study on measuring the number concentration of colloidal gold nanoparticles
Journal article, 2022

We describe the outcome of a large international interlaboratory study of the measurement of particle number concentration of colloidal nanoparticles, project 10 of the technical working area 34, "Nanoparticle Populations" of the Versailles Project on Advanced Materials and Standards (VAMAS). A total of 50 laboratories delivered results for the number concentration of 30 nm gold colloidal nanoparticles measured using particle tracking analysis (PTA), single particle inductively coupled plasma mass spectrometry (spICP-MS), ultraviolet-visible (UV-Vis) light spectroscopy, centrifugal liquid sedimentation (CLS) and small angle X-ray scattering (SAXS). The study provides quantitative data to evaluate the repeatability of these methods and their reproducibility in the measurement of number concentration of model nanoparticle systems following a common measurement protocol. We find that the population-averaging methods of SAXS, CLS and UV-Vis have high measurement repeatability and reproducibility, with between-labs variability of 2.6%, 11% and 1.4% respectively. However, results may be significantly biased for reasons including inaccurate material properties whose values are used to compute the number concentration. Particle-counting method results are less reproducibile than population-averaging methods, with measured between-labs variability of 68% and 46% for PTA and spICP-MS respectively. This study provides the stakeholder community with important comparative data to underpin measurement reproducibility and method validation for number concentration of nanoparticles.

Author

Caterina Minelli

National Physical Laboratory (NPL)

Magdalena Wywijas

ET Enterprises

National Physical Laboratory (NPL)

Dorota Bartczak

National Measurement Laboratory

Susana Cuello-Nunez

National Measurement Laboratory

Heidi Goenaga Infante

National Measurement Laboratory

Jerome Deumer

Physikalisch-Technische Bundesanstalt (PTB)

Christian Gollwitzer

Physikalisch-Technische Bundesanstalt (PTB)

Michael Krumrey

Physikalisch-Technische Bundesanstalt (PTB)

Karen E. Murphy

National Institute of Standards and Technology (NIST)

Monique E. Johnson

National Institute of Standards and Technology (NIST)

Antonio R. Montoro Bustos

National Institute of Standards and Technology (NIST)

Ingo H. Strenge

National Institute of Standards and Technology (NIST)

University of Siegen

Bertrand Faure

Xenocs

Peter Hoghoj

Xenocs

Vivian Tong

National Physical Laboratory (NPL)

Loic Burr

Centre suisse d'électronique et de microtechnique (CSEM)

Karin Norling

Chalmers, Biology and Biological Engineering, Chemical Biology

Other publications Research

Fredrik Höök

Chalmers, Physics, Nano and Biophysics

Other publications Research

Matthias Roesslein

Swiss Federal Laboratories for Materials Science and Technology (Empa)

Jovana Kocic

Swiss Federal Institute of Technology in Zürich (ETH)

Lyndsey Hendriks

Swiss Federal Institute of Technology in Zürich (ETH)

Tofwerk AG

Vikram Kestens

European Commission (EC)

Yannic Ramaye

European Commission (EC)

Maria C. Contreras Lopez

European Commission (EC)

Guy Auclair

European Commission (EC)

Dora Mehn

European Commission (EC)

Douglas Gilliland

European Commission (EC)

Annegret Potthoff

Fraunhofer-Gesellschaft Zur